Nitric oxide contributes to copper tolerance by influencing ROS metabolism in Arabidopsis

Andrea Peto, Nóra Lehotai, Gábor Feigl, Nóra Tugyi, Attila Ördög, Katalin Gémes, I. Tari, L. Erdei, Zsuzsanna Kolbert

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Key message: Nitric oxide improves copper tolerance via modulation of superoxide and hydrogen peroxide levels. This reflects the necessity of a well-coordinated interplay between NO and ROS during stress tolerance. Copper (Cu) excess causes toxicity and one probable consequence of this is the disturbance of cell redox state maintenance, inter alia, by reactive oxygen- (ROS) and nitrogen species (RNS). The objective of this paper was to examine the role of nitric oxide (NO) in Cu stress tolerance and its relationship with ROS in Arabidopsis. In agar-grown seedlings, concentration-dependent Cu accumulation was observed. The 5 μM Cu resulted in reduced cell viability in the NO overproducing nox1 and gsnor1-3 root tips compared to the wild-type (WT). In contrast, 25 and 50 μM Cu caused higher viability in these mutants, while in the NO-lacking nia1nia2 lower viability was detected than in the WT. The exogenous NO donor enhanced cell viability and scavenging endogenous NO decreased it in Cu-exposed WT seedlings. Besides, SNP in nia1nia2 roots led to the improvement of viability. The ascorbic acid-deficient mutants (vtc2-1, vtc2-3) possessing slightly elevated ROS levels proved to be Cu sensitive, while miox4 showing decreased ROS production was more tolerant to Cu than the WT. In nox1 and gsnor1-3, Cu did not induce superoxide formation, and H2O2 accumulation occurred only in the case of NO deficiency. Based on these, under mild stress NO intensifies cell injury, while in the case of severe Cu excess it contributes to better viability. ROS were found to be responsible for aggravation of Cu-induced damage. NO alleviates acute Cu stress via modulation of O 2 ·- and H2O2 levels reflecting the necessity of a well-coordinated interplay between NO and ROS during stress tolerance.

Original languageEnglish
Pages (from-to)1913-1923
Number of pages11
JournalPlant Cell Reports
Volume32
Issue number12
DOIs
Publication statusPublished - Dec 2013

Fingerprint

nitric oxide
copper
Arabidopsis
metabolism
viability
stress tolerance
superoxide anion
cell viability
mutants
seedlings
root tips
hydrogen peroxide
ascorbic acid
agar
cells
toxicity
oxygen
nitrogen

Keywords

  • Arabidopsis thaliana L.
  • Copper stress tolerance
  • Nitric oxide
  • Reactive oxygen species

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Peto, A., Lehotai, N., Feigl, G., Tugyi, N., Ördög, A., Gémes, K., ... Kolbert, Z. (2013). Nitric oxide contributes to copper tolerance by influencing ROS metabolism in Arabidopsis. Plant Cell Reports, 32(12), 1913-1923. https://doi.org/10.1007/s00299-013-1503-5

Nitric oxide contributes to copper tolerance by influencing ROS metabolism in Arabidopsis. / Peto, Andrea; Lehotai, Nóra; Feigl, Gábor; Tugyi, Nóra; Ördög, Attila; Gémes, Katalin; Tari, I.; Erdei, L.; Kolbert, Zsuzsanna.

In: Plant Cell Reports, Vol. 32, No. 12, 12.2013, p. 1913-1923.

Research output: Contribution to journalArticle

Peto, A, Lehotai, N, Feigl, G, Tugyi, N, Ördög, A, Gémes, K, Tari, I, Erdei, L & Kolbert, Z 2013, 'Nitric oxide contributes to copper tolerance by influencing ROS metabolism in Arabidopsis', Plant Cell Reports, vol. 32, no. 12, pp. 1913-1923. https://doi.org/10.1007/s00299-013-1503-5
Peto, Andrea ; Lehotai, Nóra ; Feigl, Gábor ; Tugyi, Nóra ; Ördög, Attila ; Gémes, Katalin ; Tari, I. ; Erdei, L. ; Kolbert, Zsuzsanna. / Nitric oxide contributes to copper tolerance by influencing ROS metabolism in Arabidopsis. In: Plant Cell Reports. 2013 ; Vol. 32, No. 12. pp. 1913-1923.
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