Nitrative stress and poly(ADP-ribose) polymerase activation in healthy and gestational diabetic pregnancies

E. M. Horváth, R. Magenheim, E. Kugler, G. Vácz, A. Szigethy, F. Lévárdi, M. Kollai, C. Szabo, Z. Lacza

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Aims/hypothesis: Increased oxidative-nitrosative stress, poly(ADP-ribose) polymerase (PARP) activation and subsequent cellular damage play important roles in the complications of both diabetes mellitus and pregnancy. Our aim was to investigate nitrative stress and PARP activity levels during normal and gestational diabetic (GDM) pregnancy in both maternal and fetal tissues. Methods: Blood samples were collected during pregnancy (weeks 16-29 and 36-40), and placental and umbilical cord tissues were harvested after delivery from healthy volunteers and GDM patients subjected to a carbohydrate-restricted diet or insulin treatment. Immunohistochemical staining was performed on leucocytes and tissue sections using anti-nitrotyrosine (NT), anti-poly(ADP-ribose) (PAR) and anti-apoptosis inducing factor antibodies. Results: In healthy pregnancies the intensity of NT and PAR staining of leucocytes correlated positively with gestational week (R 2∈=∈0.43, p∈2∈=∈0.49, p∈2∈=∈0.49, p∈

Original languageEnglish
Pages (from-to)1935-1943
Number of pages9
JournalDiabetologia
Volume52
Issue number9
DOIs
Publication statusPublished - Sep 2009

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Poly(ADP-ribose) Polymerases
Poly Adenosine Diphosphate Ribose
Pregnancy
Leukocytes
Carbohydrate-Restricted Diet
Apoptosis Inducing Factor
Staining and Labeling
Pregnancy in Diabetics
Umbilical Cord
Diabetes Complications
Healthy Volunteers
Oxidative Stress
Fetus
Mothers
Insulin
Antibodies
3-nitrotyrosine
Therapeutics

Keywords

  • Circulating leucocytes
  • Gestational diabetes
  • Insulin treatment
  • Peroxynitrite
  • Poly(ADP-ribose) polymerase

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Nitrative stress and poly(ADP-ribose) polymerase activation in healthy and gestational diabetic pregnancies. / Horváth, E. M.; Magenheim, R.; Kugler, E.; Vácz, G.; Szigethy, A.; Lévárdi, F.; Kollai, M.; Szabo, C.; Lacza, Z.

In: Diabetologia, Vol. 52, No. 9, 09.2009, p. 1935-1943.

Research output: Contribution to journalArticle

Horváth, EM, Magenheim, R, Kugler, E, Vácz, G, Szigethy, A, Lévárdi, F, Kollai, M, Szabo, C & Lacza, Z 2009, 'Nitrative stress and poly(ADP-ribose) polymerase activation in healthy and gestational diabetic pregnancies', Diabetologia, vol. 52, no. 9, pp. 1935-1943. https://doi.org/10.1007/s00125-009-1435-3
Horváth, E. M. ; Magenheim, R. ; Kugler, E. ; Vácz, G. ; Szigethy, A. ; Lévárdi, F. ; Kollai, M. ; Szabo, C. ; Lacza, Z. / Nitrative stress and poly(ADP-ribose) polymerase activation in healthy and gestational diabetic pregnancies. In: Diabetologia. 2009 ; Vol. 52, No. 9. pp. 1935-1943.
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