Nicotinic acid and pyridoxine modulate arachidonic acid metabolism in vitro and ex vivo in man

Virpi Saareks, I. Muchá, Eeva Sievi, Asko Riutta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The in vitro effects of nicotinic acid (10-1000 μM), pyridoxine (0.1- 500 μM) and pyridoxal-5'-phosphate (0.1500 μM) and the ex vivo effects of nicotinic acid (2500 mg orally during 12 h) and pyridoxine (600 mg orally daily for seven days) on arachidonic acid metabolism were investigated in calcium ionophore A23187 (calcimycin)-stimulated human whole blood. In vitro nicotinic acid stimulated prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis. Pyridoxine at all concentrations and pyridoxal-5'-phosphate at the highest concentration stimulated prostaglandin E2 and thromboxane B2 production, but had no effect on leukotriene E4 synthesis. Nicotinic acid treatment increased ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis to 185%, 165% and 175% of the initial values, respectively. In the pyridoxine-treated subjects, ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis was decreased after seven days to 75%, 65% and 45% of the initial values, respectively. In the present study the effects of nicotinic acid on the 5-lipoxygenase pathway in arachidonic acid metabolism were studied for the first time and the drug was found to stimulate this pathway in vitro and ex vivo. In vitro pyridoxine and pyridoxal-5'-phosphate had no effect on the 5-lipoxygenase pathway. The inhibition of leukotriene synthesis by pyridoxine ex vivo might be of therapeutic importance.

Original languageEnglish
Pages (from-to)274-280
Number of pages7
JournalPharmacology and Toxicology
Volume84
Issue number6
Publication statusPublished - 1999

Fingerprint

Pyridoxine
Niacin
Leukotriene E4
Thromboxane B2
Arachidonic Acid
Metabolism
Dinoprostone
Pyridoxal Phosphate
Arachidonate 5-Lipoxygenase
Calcimycin
Calcium Ionophores
Leukotrienes
In Vitro Techniques
Blood
Therapeutics
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Nicotinic acid and pyridoxine modulate arachidonic acid metabolism in vitro and ex vivo in man. / Saareks, Virpi; Muchá, I.; Sievi, Eeva; Riutta, Asko.

In: Pharmacology and Toxicology, Vol. 84, No. 6, 1999, p. 274-280.

Research output: Contribution to journalArticle

Saareks, Virpi ; Muchá, I. ; Sievi, Eeva ; Riutta, Asko. / Nicotinic acid and pyridoxine modulate arachidonic acid metabolism in vitro and ex vivo in man. In: Pharmacology and Toxicology. 1999 ; Vol. 84, No. 6. pp. 274-280.
@article{da81340c453148e1a139f0a80317f96e,
title = "Nicotinic acid and pyridoxine modulate arachidonic acid metabolism in vitro and ex vivo in man",
abstract = "The in vitro effects of nicotinic acid (10-1000 μM), pyridoxine (0.1- 500 μM) and pyridoxal-5'-phosphate (0.1500 μM) and the ex vivo effects of nicotinic acid (2500 mg orally during 12 h) and pyridoxine (600 mg orally daily for seven days) on arachidonic acid metabolism were investigated in calcium ionophore A23187 (calcimycin)-stimulated human whole blood. In vitro nicotinic acid stimulated prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis. Pyridoxine at all concentrations and pyridoxal-5'-phosphate at the highest concentration stimulated prostaglandin E2 and thromboxane B2 production, but had no effect on leukotriene E4 synthesis. Nicotinic acid treatment increased ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis to 185{\%}, 165{\%} and 175{\%} of the initial values, respectively. In the pyridoxine-treated subjects, ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis was decreased after seven days to 75{\%}, 65{\%} and 45{\%} of the initial values, respectively. In the present study the effects of nicotinic acid on the 5-lipoxygenase pathway in arachidonic acid metabolism were studied for the first time and the drug was found to stimulate this pathway in vitro and ex vivo. In vitro pyridoxine and pyridoxal-5'-phosphate had no effect on the 5-lipoxygenase pathway. The inhibition of leukotriene synthesis by pyridoxine ex vivo might be of therapeutic importance.",
author = "Virpi Saareks and I. Much{\'a} and Eeva Sievi and Asko Riutta",
year = "1999",
language = "English",
volume = "84",
pages = "274--280",
journal = "Basic and Clinical Pharmacology and Toxicology",
issn = "1742-7835",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Nicotinic acid and pyridoxine modulate arachidonic acid metabolism in vitro and ex vivo in man

AU - Saareks, Virpi

AU - Muchá, I.

AU - Sievi, Eeva

AU - Riutta, Asko

PY - 1999

Y1 - 1999

N2 - The in vitro effects of nicotinic acid (10-1000 μM), pyridoxine (0.1- 500 μM) and pyridoxal-5'-phosphate (0.1500 μM) and the ex vivo effects of nicotinic acid (2500 mg orally during 12 h) and pyridoxine (600 mg orally daily for seven days) on arachidonic acid metabolism were investigated in calcium ionophore A23187 (calcimycin)-stimulated human whole blood. In vitro nicotinic acid stimulated prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis. Pyridoxine at all concentrations and pyridoxal-5'-phosphate at the highest concentration stimulated prostaglandin E2 and thromboxane B2 production, but had no effect on leukotriene E4 synthesis. Nicotinic acid treatment increased ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis to 185%, 165% and 175% of the initial values, respectively. In the pyridoxine-treated subjects, ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis was decreased after seven days to 75%, 65% and 45% of the initial values, respectively. In the present study the effects of nicotinic acid on the 5-lipoxygenase pathway in arachidonic acid metabolism were studied for the first time and the drug was found to stimulate this pathway in vitro and ex vivo. In vitro pyridoxine and pyridoxal-5'-phosphate had no effect on the 5-lipoxygenase pathway. The inhibition of leukotriene synthesis by pyridoxine ex vivo might be of therapeutic importance.

AB - The in vitro effects of nicotinic acid (10-1000 μM), pyridoxine (0.1- 500 μM) and pyridoxal-5'-phosphate (0.1500 μM) and the ex vivo effects of nicotinic acid (2500 mg orally during 12 h) and pyridoxine (600 mg orally daily for seven days) on arachidonic acid metabolism were investigated in calcium ionophore A23187 (calcimycin)-stimulated human whole blood. In vitro nicotinic acid stimulated prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis. Pyridoxine at all concentrations and pyridoxal-5'-phosphate at the highest concentration stimulated prostaglandin E2 and thromboxane B2 production, but had no effect on leukotriene E4 synthesis. Nicotinic acid treatment increased ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis to 185%, 165% and 175% of the initial values, respectively. In the pyridoxine-treated subjects, ex vivo prostaglandin E2, thromboxane B2 and leukotriene E4 synthesis was decreased after seven days to 75%, 65% and 45% of the initial values, respectively. In the present study the effects of nicotinic acid on the 5-lipoxygenase pathway in arachidonic acid metabolism were studied for the first time and the drug was found to stimulate this pathway in vitro and ex vivo. In vitro pyridoxine and pyridoxal-5'-phosphate had no effect on the 5-lipoxygenase pathway. The inhibition of leukotriene synthesis by pyridoxine ex vivo might be of therapeutic importance.

UR - http://www.scopus.com/inward/record.url?scp=0032977431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032977431&partnerID=8YFLogxK

M3 - Article

C2 - 10401729

AN - SCOPUS:0032977431

VL - 84

SP - 274

EP - 280

JO - Basic and Clinical Pharmacology and Toxicology

JF - Basic and Clinical Pharmacology and Toxicology

SN - 1742-7835

IS - 6

ER -