Nicotine stereoisomers and cotinine stimulate prostaglandin E2 but inhibit thromboxane B2 and leukotriene E4 synthesis in whole blood

Virpi Saareks, István Mucha, Eeva Sievi, Heikki Vapaatalo, Asko Riutta

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effects of (-)-nicotine (0.0005-500 μM), (+)-nicotine (0.0005-50 μM) and (-)-cotinine (0.0005-500 μM) on arachidonic acid metabolism were investigated in Ca2+ ionophore A23187 (calcimycin)-stimulated human whole blood in vitro. (-)-Nicotine and (-)-cotinine stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis, as has been observed previously in A23187-stimulated polymorphonuclear leukocytes and platelet-rich plasma [Saareks, V., Riutta, A., Mucha, I., Alanko, J., Vapaatalo, H., 1993. Nicotine and cotinine modulate eicosanoid production in human leukocytes and platelet rich plasma. Eur. J. Pharmacol., 248, 345-349.]. (+)-Nicotine also stimulated prostaglandin E2 but inhibited thromboxane B2 synthesis. High concentrations of (-)-nicotine and (-)-cotinine and even nanomolar concentrations of (+)-nicotine inhibited leukotriene E4 synthesis. These results indicate that (-)-nicotine and (-)-cotinine stimulate cyclooxygenase but inhibit thromboxane synthase and 5-lipoxygenase in whole blood in vitro. (+)-Nicotine is capable of affecting in the same direction as well. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalEuropean Journal of Pharmacology
Volume353
Issue number1
DOIs
Publication statusPublished - Jul 17 1998

Keywords

  • Blood
  • Cotinine
  • Leukotrienes
  • Nicotine
  • Prostaglandin
  • Thromboxane

ASJC Scopus subject areas

  • Pharmacology

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