Nicotine-induced alteration in Tyr-Gly-Gly and Met-enkephalin in discrete brain nuclei reflects altered enkephalin neuron activity

Abdulghani A. Houdi, Krystyna Pierzchala, Lesley Marson, M. Palkóvits, Glen R. Van Loon

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Nicotine acts in CNS, but the pathways and mechanisms of its action are poorly understood. Recent studies suggest an interaction between brain nicotinic receptors and endogenous opioid peptides. Acute administration of nicotine may alter enkephalin release without affecting brain enkephalin level. Tyr-Gly-Gly has been shown previously to be an extraneuronal metabolite of opioid peptides derived from proenkephalin A. Concentrations of Tyr-Gly-Gly in brain were used to provide an index of enkephalin release in vivo. Thus we examined the thesis that nicotine alters brain neuronal enkephalin release, by measuring Tyr-Gly-Gly levels in specific brain nuclei from rats treated with nicotine 0.3 mg/kg SC 10 min before decapitation. Of 30 brain regions investigated, acute nicotine increased Tyr-Gly-Gly immunoreactivity in nucleus accumbens and in lower brain stem areas including dorsal raphe, pontine reticular formation, gigantocellular reticular formation, locus coeruleus, sensory trigeminal nucleus and the caudal part of ventrolateral medulla oblongata. Concomitantly, nicotine produced a significant decrease in native Met-enkephalin in central amygdala, flocculo-nodular lobe of cerebellum, caudal part of the ventrolateral medulla and intermediolateral cell column of the spinal cord. It is probable that the effects of nicotine to increase Tyr-Gly-Gly and alter Met-enkephalin concentration are mediated by nicotine-induced release of enkephalin at these brain sites. Furthermore, some of the physiologic and pharmacologic effects of nicotine may be mediated by such enkephalin release.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalPeptides
Volume12
Issue number1
DOIs
Publication statusPublished - 1991

Fingerprint

tyrosyl-glycyl-glycine
Methionine Enkephalin
Enkephalins
Nicotine
Neurons
Brain
Opioid Peptides
Trigeminal Caudal Nucleus
Decapitation
Medulla Oblongata
Reticular Formation
Locus Coeruleus
Nucleus Accumbens
Nicotinic Receptors
Metabolites
Cerebellum
Brain Stem
Rats
Spinal Cord

Keywords

  • Met-Enkephalin
  • Neuropeptide release
  • Nicotine
  • Opioid peptide, release
  • Tripeptide Tyr-Gly-Gly

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Nicotine-induced alteration in Tyr-Gly-Gly and Met-enkephalin in discrete brain nuclei reflects altered enkephalin neuron activity. / Houdi, Abdulghani A.; Pierzchala, Krystyna; Marson, Lesley; Palkóvits, M.; Van Loon, Glen R.

In: Peptides, Vol. 12, No. 1, 1991, p. 161-166.

Research output: Contribution to journalArticle

Houdi, Abdulghani A. ; Pierzchala, Krystyna ; Marson, Lesley ; Palkóvits, M. ; Van Loon, Glen R. / Nicotine-induced alteration in Tyr-Gly-Gly and Met-enkephalin in discrete brain nuclei reflects altered enkephalin neuron activity. In: Peptides. 1991 ; Vol. 12, No. 1. pp. 161-166.
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