NHERF2 is crucial in ERM phosphorylation in pulmonary endothelial cells

Anita Boratkó, C. Csortos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: EBP50 and NHERF2 adaptor proteins are incriminated in various signaling pathways of the cell. They can bind ERM proteins and mediate ERM-membrane protein interactions. Results: Binding of ERM to EBP50 and NHERF2 was compared in pulmonary artery endothelial cells by immunoprecipitation. NHERF2 associates with all three ERM, but EBP50 appeared to be a weak binding partner if at all. Furthermore, we detected co-localization of NHERF2 and phospho-ERM at the cell membrane and in the filopodia of dividing cells. Silencing of NHERF2 prevented agonist or angiogenesis induced phosphorylation of ERM, while overexpression of the adaptor elevated the phosphorylation level of ERM, likely catalyzed by Rho kinase 2, which co-immunoprecipitated with NHERF2/ERM in control EC, but did not bind to ERM in NHERF2 depleted cells. Dependence of ERM phosphorylation on NHERF2 was also shown in Matrigel tube formation assay, and NHERF2 was proved to be important in angiogenesis as well. Furthermore, when NHERF2 was depleted or cells were overexpressing a mutant form of NHERF2 unable to bind ERM, we found attenuated cell attachment with ECIS measurements, while it was supported by overexpression of wild type NHERF2. Conclusions: Pivotal role of NHERF2 in the phosphorylation process of ERM in pulmonary artery endothelial cells is shown. We propose that NHERF2 provides a common anchoring surface for ERM and Rho kinase 2. Our results demonstrate the essential role of NHERF2 in endothelial cell adhesion/migration and angiogenesis.

Original languageEnglish
Article number99
JournalCell Communication and Signaling
Volume11
Issue number1
DOIs
Publication statusPublished - Dec 23 2013

Fingerprint

Phosphorylation
Endothelial cells
Endothelial Cells
rho-Associated Kinases
Lung
Pulmonary Artery
Cell adhesion
Cell membranes
Pseudopodia
Assays
Membrane Proteins
Proteins
Immunoprecipitation
Cell Adhesion
Cell Movement
Cell Membrane

Keywords

  • Angiogenesis
  • Endothelial cells
  • ERM proteins
  • NHERF2

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biochemistry
  • Medicine(all)

Cite this

NHERF2 is crucial in ERM phosphorylation in pulmonary endothelial cells. / Boratkó, Anita; Csortos, C.

In: Cell Communication and Signaling, Vol. 11, No. 1, 99, 23.12.2013.

Research output: Contribution to journalArticle

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