NGS of virus-derived small RNAs as a diagnostic method used to determine viromes of Hungarian Vineyards

Nikoletta Czotter, Janos Molnar, Emese Szabó, Emese Demian, Levente Kontra, Ivett Baksa, Gyorgy Szittya, Laszlo Kocsis, Tamas Deak, Gyorgy Bisztray, G. Tusnády, J. Burgyán, E. Várallyay

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10 Citations (Scopus)

Abstract

As virus diseases cannot be controlled by traditional plant protection methods, the risk of their spread have to be minimized on vegetatively propagated plants, such as grapevine. Metagenomic approaches used for virus diagnostics offer a unique opportunity to reveal the presence of all viral pathogens in the investigated plant, which is why their application can reduce the risk of using infected material for a new plantation. Here we used a special branch, deep sequencing of virus-derived small RNAs, of this high-throughput method for virus diagnostics, and determined viromes of vineyards in Hungary. With NGS of virus-derived small RNAs we could detect not only the viruses tested routinely, but also new ones, which had never been described in Hungary before. Virus presence did not correlate with the age of the plantation, moreover phylogenetic analysis of the identified virus isolates suggests that infections are mostly caused by the use of infected propagating material. Our results, validated by other molecular methods, raised further questions to be answered before this method can be introduced as a routine, reliable test for grapevine virus diagnostics.

Original languageEnglish
Article number122
JournalFrontiers in Microbiology
Volume9
Issue numberFEB
DOIs
Publication statusPublished - Feb 6 2018

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Keywords

  • Diagnostics
  • Grapevine
  • Metagenomics
  • NGS
  • Small RNA
  • Vineyard
  • Virome
  • Virus

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Czotter, N., Molnar, J., Szabó, E., Demian, E., Kontra, L., Baksa, I., Szittya, G., Kocsis, L., Deak, T., Bisztray, G., Tusnády, G., Burgyán, J., & Várallyay, E. (2018). NGS of virus-derived small RNAs as a diagnostic method used to determine viromes of Hungarian Vineyards. Frontiers in Microbiology, 9(FEB), [122]. https://doi.org/10.3389/fmicb.2018.00122