Következo generációs szekvenálási technológiák kifejlodése és alkalmazásai

Translated title of the contribution: Next generation sequencing technologies (NGST) development and applications

Zsuzsanna Mihály, B. Györffy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the past ten years the development of next generation sequencing technologies brought a new era in the field of quick and efficient DNA sequencing. In our study we give an overview of the methodological achievements from Sanger's chain-termination sequencing in 1975 to those allowing real-time DNA sequencing today. Sequencing methods that utilize clonal amplicons for parallel multistrand sequencing comprise the basics of currently available next generation sequencing techniques. Nowadays next generation sequencing is mainly used for basic research in functional genomics, providing quintessential information in the meta-analyses of data from signal transduction pathways, onthologies, proteomics and metabolomics. Although next generation sequencing is yet sparsely used in clinical practice, cardiology, oncology and epidemiology already show an immense need for the additional knowledge obtained by this new technology. The main barrier of its spread is the lack of standardization of analysis evaluation methods, which obscure objective assessment of the results. Orv. Hetil., 2011, 152, 55-62.

Original languageHungarian
Pages (from-to)55-62
Number of pages8
JournalOrvosi Hetilap
Volume152
Issue number2
DOIs
Publication statusPublished - Jan 1 2011

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DNA Sequence Analysis
Technology
Metabolomics
Genomics
Cardiology
Proteomics
Meta-Analysis
Signal Transduction
Epidemiology
Research
Industrial Development

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Következo generációs szekvenálási technológiák kifejlodése és alkalmazásai. / Mihály, Zsuzsanna; Györffy, B.

In: Orvosi Hetilap, Vol. 152, No. 2, 01.01.2011, p. 55-62.

Research output: Contribution to journalArticle

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