Newcastle disease virus (NDV) induces protein oxidation and nitration in brain and liver of chicken: Ameliorative effect of Vitamin E

Kadiam C. Venkata Subbaiah, Lokanatha Valluru, Wudayagiri Rajendra, Chiteti Ramamurthy, Chinnasamy Thirunavukkarusu, S. Rajagopal

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The present study was aimed at investigating the therapeutic efficacy of Vitamin E on oxidative injury in brain and liver of Newcastle disease virus (NDV) challenged chickens. We have analyzed the xanthine oxidase (XOD) activity; uric acid (UA) levels and superoxide radical generation by using electron spin resonance spectroscopy. Further, protein oxidation, nitration and apoptosis were evaluated in the brain and liver of the control, NDV-infected and NDV + Vit. E treated groups. A significant elevation was observed in XOD activity and UA levels in brain (p <0.001) and liver (p <0.05) of NDV infected birds when compared to controls. Further, significant increase in the production of superoxides, enhanced intracellular protein carbonyls and nitrates were observed in the brain and liver of NDV-infected birds over healthy subjects. Apoptosis studies also suggested that a larger number of TUNEL positive cells were observed in brain and a moderately in liver of NDV-infected chickens. However, all these perturbations were significantly ameliorated in NDV + Vit. E treated chickens as compared to NDV-infected birds. Taken together, our results suggested that NDV-induced neuronal and hepatic damage at least in part mediates oxidative stress and on the other hand, supplementation of Vitamin E mitigates NDV-induced oxidative damage thereby protects brain and liver of chickens. These findings could provide new insights into the understanding of NDV pathogenesis and therapeutic effects of dietary antioxidants.

Original languageEnglish
Pages (from-to)97-106
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume64
DOIs
Publication statusPublished - 2015

Fingerprint

Nitration
Newcastle disease virus
Vitamin E
Viruses
Liver
Chickens
Brain
Oxidation
Proteins
Birds
Liver Diseases
Xanthine Oxidase
Uric Acid
Superoxides
Apoptosis
Electron spin resonance spectroscopy
Disease control
Oxidative stress
In Situ Nick-End Labeling
Electron Spin Resonance Spectroscopy

Keywords

  • Apoptosis
  • Electron spin resonance studies
  • Immuno fluorescence studies
  • Newcastle disease virus
  • Protein nitration
  • Protein oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Newcastle disease virus (NDV) induces protein oxidation and nitration in brain and liver of chicken : Ameliorative effect of Vitamin E. / Venkata Subbaiah, Kadiam C.; Valluru, Lokanatha; Rajendra, Wudayagiri; Ramamurthy, Chiteti; Thirunavukkarusu, Chinnasamy; Rajagopal, S.

In: International Journal of Biochemistry and Cell Biology, Vol. 64, 2015, p. 97-106.

Research output: Contribution to journalArticle

Venkata Subbaiah, Kadiam C. ; Valluru, Lokanatha ; Rajendra, Wudayagiri ; Ramamurthy, Chiteti ; Thirunavukkarusu, Chinnasamy ; Rajagopal, S. / Newcastle disease virus (NDV) induces protein oxidation and nitration in brain and liver of chicken : Ameliorative effect of Vitamin E. In: International Journal of Biochemistry and Cell Biology. 2015 ; Vol. 64. pp. 97-106.
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