New Water-Soluble Copper(II) Complexes with Morpholine-Thiosemicarbazone Hybrids: Insights into the Anticancer and Antibacterial Mode of Action

Kateryna Ohui, Eleonora Afanasenko, Felix Bacher, Rachel Lim Xue Ting, Ayesha Zafar, Núria Blanco-Cabra, Eduard Torrents, Orsolya Dömötör, Nóra V. May, Denisa Darvasiova, Éva A. Enyedy, Ana Popović-Bijelić, Jóhannes Reynisson, Peter Rapta, Maria V. Babak, Giorgia Pastorin, Vladimir B. Arion

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13 Citations (Scopus)

Abstract

Six morpholine-(iso)thiosemicarbazone hybrids HL 1 -HL 6 and their Cu(II) complexes with good-to-moderate solubility and stability in water were synthesized and characterized. Cu(II) complexes [Cu(L 1-6 )Cl] (1-6) formed weak dimeric associates in the solid state, which did not remain intact in solution as evidenced by ESI-MS. The lead proligands and Cu(II) complexes displayed higher antiproliferative activity in cancer cells than triapine. In addition, complexes 2-5 were found to specifically inhibit the growth of Gram-positive bacteria Staphylococcus aureus with MIC 50 values at 2-5 μg/mL. Insights into the processes controlling intracellular accumulation and mechanism of action were investigated for 2 and 5, including the role of ribonucleotide reductase (RNR) inhibition, endoplasmic reticulum stress induction, and regulation of other cancer signaling pathways. Their ability to moderately inhibit R2 RNR protein in the presence of dithiothreitol is likely related to Fe chelating properties of the proligands liberated upon reduction.

Original languageEnglish
Pages (from-to)512-530
Number of pages19
JournalJournal of Medicinal Chemistry
Volume62
Issue number2
DOIs
Publication statusPublished - Jan 24 2019

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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    Ohui, K., Afanasenko, E., Bacher, F., Ting, R. L. X., Zafar, A., Blanco-Cabra, N., Torrents, E., Dömötör, O., May, N. V., Darvasiova, D., Enyedy, É. A., Popović-Bijelić, A., Reynisson, J., Rapta, P., Babak, M. V., Pastorin, G., & Arion, V. B. (2019). New Water-Soluble Copper(II) Complexes with Morpholine-Thiosemicarbazone Hybrids: Insights into the Anticancer and Antibacterial Mode of Action. Journal of Medicinal Chemistry, 62(2), 512-530. https://doi.org/10.1021/acs.jmedchem.8b01031