New type of nerve-mediated cholinergic contractions of the guinea-pig small intestine and its selective blockade by capsaicin

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Abstract

1. Electrical stimulation (2-50Hz) of mesenteric nerves of the guinea-pig isolated ileum resulted in contraction of preparations pretreated with adrenergic neuron blocking agents (guanethidine, bretylium), or on preparations obtained from animals pretreated with reserpine. Stimulation at low frequencies (2-10 Hz) also caused contraction in untreated preparations. 2. The response was abolished by hyoscine (1 ×10-7-1×10-6 g/ml) or morphine (2×10-7 g/ml). However, previous bilateral vagotomy, hexamethonium (1×10-4 g/ml), mecamylamine (1×10-5 g/ml), or desensitization of the gut to 5-HT caused practically no inhibition. 3. Capsaicin inhibited or abolished (IC50 1.5 ×10-8 g/ml) the contraction elicited by stimulation of mesenteric nerves in an irreversible manner. The drug did not inhibit the contraction to field stimulation of the postganglionic cholinergic fibres. 4. Neither the contraction of the duodenum to stimulation of the preganglionic vagal fibres, nor the adrenergic inhibition elicited by periarterial nerve stimulation were influenced by capsaicin. 5. It is concluded that the cholinergic response described above is neither parasympathetic in origin nor can it explained on the basis of a cholinergic mechanism in adrenergic neurotransmission (Burn's theory). A hypothesis is put forward that nerve fibres characterized by their specific sensitivity to capsaicin, presumably originating from sensory neurons excite cholinergic neurons of the myenteric plexus.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume305
Issue number1
DOIs
Publication statusPublished - Oct 1978

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Capsaicin
Cholinergic Agents
Small Intestine
Guinea Pigs
Adrenergic Agents
Cholinergic Fibers
Mecamylamine
Adrenergic Fibers
Guanethidine
Myenteric Plexus
Hexamethonium
Cholinergic Neurons
Adrenergic Antagonists
Scopolamine Hydrobromide
Vagotomy
Reserpine
Sensory Receptor Cells
Ileum
Nerve Fibers
Duodenum

Keywords

  • Capsaicin
  • Cholinergic mechanism
  • Ileum innervation
  • Periarterial mesenteric nerves
  • Sensory fibres

ASJC Scopus subject areas

  • Pharmacology

Cite this

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title = "New type of nerve-mediated cholinergic contractions of the guinea-pig small intestine and its selective blockade by capsaicin",
abstract = "1. Electrical stimulation (2-50Hz) of mesenteric nerves of the guinea-pig isolated ileum resulted in contraction of preparations pretreated with adrenergic neuron blocking agents (guanethidine, bretylium), or on preparations obtained from animals pretreated with reserpine. Stimulation at low frequencies (2-10 Hz) also caused contraction in untreated preparations. 2. The response was abolished by hyoscine (1 ×10-7-1×10-6 g/ml) or morphine (2×10-7 g/ml). However, previous bilateral vagotomy, hexamethonium (1×10-4 g/ml), mecamylamine (1×10-5 g/ml), or desensitization of the gut to 5-HT caused practically no inhibition. 3. Capsaicin inhibited or abolished (IC50 1.5 ×10-8 g/ml) the contraction elicited by stimulation of mesenteric nerves in an irreversible manner. The drug did not inhibit the contraction to field stimulation of the postganglionic cholinergic fibres. 4. Neither the contraction of the duodenum to stimulation of the preganglionic vagal fibres, nor the adrenergic inhibition elicited by periarterial nerve stimulation were influenced by capsaicin. 5. It is concluded that the cholinergic response described above is neither parasympathetic in origin nor can it explained on the basis of a cholinergic mechanism in adrenergic neurotransmission (Burn's theory). A hypothesis is put forward that nerve fibres characterized by their specific sensitivity to capsaicin, presumably originating from sensory neurons excite cholinergic neurons of the myenteric plexus.",
keywords = "Capsaicin, Cholinergic mechanism, Ileum innervation, Periarterial mesenteric nerves, Sensory fibres",
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T1 - New type of nerve-mediated cholinergic contractions of the guinea-pig small intestine and its selective blockade by capsaicin

AU - Szolcsányi, J.

AU - Barthó, L.

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N2 - 1. Electrical stimulation (2-50Hz) of mesenteric nerves of the guinea-pig isolated ileum resulted in contraction of preparations pretreated with adrenergic neuron blocking agents (guanethidine, bretylium), or on preparations obtained from animals pretreated with reserpine. Stimulation at low frequencies (2-10 Hz) also caused contraction in untreated preparations. 2. The response was abolished by hyoscine (1 ×10-7-1×10-6 g/ml) or morphine (2×10-7 g/ml). However, previous bilateral vagotomy, hexamethonium (1×10-4 g/ml), mecamylamine (1×10-5 g/ml), or desensitization of the gut to 5-HT caused practically no inhibition. 3. Capsaicin inhibited or abolished (IC50 1.5 ×10-8 g/ml) the contraction elicited by stimulation of mesenteric nerves in an irreversible manner. The drug did not inhibit the contraction to field stimulation of the postganglionic cholinergic fibres. 4. Neither the contraction of the duodenum to stimulation of the preganglionic vagal fibres, nor the adrenergic inhibition elicited by periarterial nerve stimulation were influenced by capsaicin. 5. It is concluded that the cholinergic response described above is neither parasympathetic in origin nor can it explained on the basis of a cholinergic mechanism in adrenergic neurotransmission (Burn's theory). A hypothesis is put forward that nerve fibres characterized by their specific sensitivity to capsaicin, presumably originating from sensory neurons excite cholinergic neurons of the myenteric plexus.

AB - 1. Electrical stimulation (2-50Hz) of mesenteric nerves of the guinea-pig isolated ileum resulted in contraction of preparations pretreated with adrenergic neuron blocking agents (guanethidine, bretylium), or on preparations obtained from animals pretreated with reserpine. Stimulation at low frequencies (2-10 Hz) also caused contraction in untreated preparations. 2. The response was abolished by hyoscine (1 ×10-7-1×10-6 g/ml) or morphine (2×10-7 g/ml). However, previous bilateral vagotomy, hexamethonium (1×10-4 g/ml), mecamylamine (1×10-5 g/ml), or desensitization of the gut to 5-HT caused practically no inhibition. 3. Capsaicin inhibited or abolished (IC50 1.5 ×10-8 g/ml) the contraction elicited by stimulation of mesenteric nerves in an irreversible manner. The drug did not inhibit the contraction to field stimulation of the postganglionic cholinergic fibres. 4. Neither the contraction of the duodenum to stimulation of the preganglionic vagal fibres, nor the adrenergic inhibition elicited by periarterial nerve stimulation were influenced by capsaicin. 5. It is concluded that the cholinergic response described above is neither parasympathetic in origin nor can it explained on the basis of a cholinergic mechanism in adrenergic neurotransmission (Burn's theory). A hypothesis is put forward that nerve fibres characterized by their specific sensitivity to capsaicin, presumably originating from sensory neurons excite cholinergic neurons of the myenteric plexus.

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