New set-up for capillary isoelectric focusing in uncoated capillaries

Ferenc Kilár, Ákos Végvári, Andor Mód

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a 'sandwich' ampholyte-sample-ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the analysis of hemoglobin variants. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)349-360
Number of pages12
JournalJournal of Chromatography A
Volume813
Issue number2
DOIs
Publication statusPublished - Jul 17 1998

Keywords

  • Dyes
  • Haemoglobin
  • Injection methods
  • Isoelectric focusing
  • Nitrophenols
  • Sandwich injection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'New set-up for capillary isoelectric focusing in uncoated capillaries'. Together they form a unique fingerprint.

  • Cite this