New phenotypic, functional and electrophysiological characteristics of KG-1 cells

György Hajas, Emese Zsiros, Tünde László, Péter Hajdú, Sándor Somodi, Bence Réthi, P. Gogolák, Katalin Ludányi, G. Panyi, E. Rajnavolgyi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3β chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca2+-gated potassium conductance suggesting the presence of KCa channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.

Original languageEnglish
Pages (from-to)97-106
Number of pages10
JournalImmunology Letters
Volume92
Issue number1-2
DOIs
Publication statusPublished - Mar 29 2004

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Dendritic Cells
Myeloid Cells
Ionomycin
CCR7 Receptors
Leukemia, Erythroblastic, Acute
Chemokine Receptors
Patch-Clamp Techniques
Antigen-Presenting Cells
Surface Antigens
Ion Channels
Chemokines
Cell Movement
Monocytes
Potassium
Cell Line
Membranes
Population

Keywords

  • APC, antigen presenting cell
  • DC, dendritic cell
  • GM-CSF, granulocyte-monocyte-colony stimulating factor
  • HSC, hematopoietic stem cell
  • LC, Langerhans cell
  • PMA, phorbol-myristil-acetate
  • TNF-α, tumor necrosis factor-α

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

New phenotypic, functional and electrophysiological characteristics of KG-1 cells. / Hajas, György; Zsiros, Emese; László, Tünde; Hajdú, Péter; Somodi, Sándor; Réthi, Bence; Gogolák, P.; Ludányi, Katalin; Panyi, G.; Rajnavolgyi, E.

In: Immunology Letters, Vol. 92, No. 1-2, 29.03.2004, p. 97-106.

Research output: Contribution to journalArticle

Hajas G, Zsiros E, László T, Hajdú P, Somodi S, Réthi B et al. New phenotypic, functional and electrophysiological characteristics of KG-1 cells. Immunology Letters. 2004 Mar 29;92(1-2):97-106. https://doi.org/10.1016/j.imlet.2003.11.021
Hajas, György ; Zsiros, Emese ; László, Tünde ; Hajdú, Péter ; Somodi, Sándor ; Réthi, Bence ; Gogolák, P. ; Ludányi, Katalin ; Panyi, G. ; Rajnavolgyi, E. / New phenotypic, functional and electrophysiological characteristics of KG-1 cells. In: Immunology Letters. 2004 ; Vol. 92, No. 1-2. pp. 97-106.
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AU - Somodi, Sándor

AU - Réthi, Bence

AU - Gogolák, P.

AU - Ludányi, Katalin

AU - Panyi, G.

AU - Rajnavolgyi, E.

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AB - Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3β chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca2+-gated potassium conductance suggesting the presence of KCa channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.

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