New nitroxide reagents for the selective spin-labelling at the guanidino moiety of arginine residues in peptides and proteins

O. H. Hankovszky, K. Hideg, E. v. Goldammer, E. Matuszak, H. Kolkenbrock, H. Tschesche, H. R. Wenzel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The synthesis of three glyoxal derivatives carrying a nitroxide moiety was achieved by the α-oxidation of the corresponding methyl ketones using selenium dioxide. These reagents appear to be useful for the specific spin-labelling at arginine side-chains. Sixteen peptides and proteins were successfully labelled and the rotational correlation times, as deduced from ESR spectra, were correlated with the molecular weights of the target macromolecules.

Original languageEnglish
Pages (from-to)152-155
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume916
Issue number1
DOIs
Publication statusPublished - Nov 5 1987

Keywords

  • ESR
  • Glyoxal reagent
  • Guanidino group
  • Nitroxide
  • Protein modification, Spin labeling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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