New methods for the identification of efflux mediated MDR bacteria, genetic assessment of regulators and efflux pump constituents, characterization of efflux systems and screening for inhibitors of efflux pumps

M. Viveiros, M. Martins, I. Couto, L. Rodrigues, G. Spengler, A. Martins, J. E. Kristiansen, J. Molnar, L. Amaral

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have developed a number of methods that identify efflux pump mediated multi-drug resistant bacteria, characterize efflux systems and screen for inhibitors of efflux pumps. These approaches were complemented by the quantification of the expression of genes that regulate and code for constituents of efflux pumps. The methods described are easy to use, reproducible and for the most part, require instrumentation normally present in a clinical bacteriology laboratory. Because each method provides good reproducibility, they lend themselves for inter-laboratory use.

Original languageEnglish
Pages (from-to)760-778
Number of pages19
JournalCurrent Drug Targets
Volume9
Issue number9
DOIs
Publication statusPublished - 2008

Fingerprint

Bacteria
Screening
Pumps
Bacteriology
Genes
Gene Expression
Pharmaceutical Preparations

Keywords

  • EB based methods
  • Efflux pump inhibitors
  • Fluorometric assay
  • Intrinsic efflux pumps
  • MDR bacteria
  • MDR efflux pumps

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

New methods for the identification of efflux mediated MDR bacteria, genetic assessment of regulators and efflux pump constituents, characterization of efflux systems and screening for inhibitors of efflux pumps. / Viveiros, M.; Martins, M.; Couto, I.; Rodrigues, L.; Spengler, G.; Martins, A.; Kristiansen, J. E.; Molnar, J.; Amaral, L.

In: Current Drug Targets, Vol. 9, No. 9, 2008, p. 760-778.

Research output: Contribution to journalArticle

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