New LC method using radioactivity detection for analysis of toxic metabolite of acetaminophen (paracetamol)

E. Hazai, E. Simon-Trompler, G. Czira, L. Vereczkey, K. Monostory

Research output: Contribution to journalArticle

Abstract

An HPLC method with UV and radioactivity detection is reported for analysis of the toxic metabolite of acetaminophen (paracetamol) in human liver samples. Acetaminophen and its oxidative metabolites were separated on on RP-18 column with aqueous NH4-ocetote:methanol mobile phase. The 14C-acetaminophen and its metabolites were monitored at 250 nm and by online-coupled radioactivity detection. HPLC-MS indicated the presence of the toxic metabolite - the new method proving suitable for its serial analysis.

Original languageEnglish
JournalChromatographia
Volume56
Issue numberSUPPL.
Publication statusPublished - 2002

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Poisons
Radioactivity
Acetaminophen
Metabolites
High Pressure Liquid Chromatography
Liver
Methanol

Keywords

  • Acetominophen (paracetamol)
  • Column liquid chromatography
  • N-acetyl-p-benzoquinone-imine
  • Radioactivity detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

New LC method using radioactivity detection for analysis of toxic metabolite of acetaminophen (paracetamol). / Hazai, E.; Simon-Trompler, E.; Czira, G.; Vereczkey, L.; Monostory, K.

In: Chromatographia, Vol. 56, No. SUPPL., 2002.

Research output: Contribution to journalArticle

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