New design method for a phase plate in super-resolution fluorescence microscopy

N. Bokor, Yoshinori Iketaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The performance of a super-resolution fluorescence depletion microscope system depends crucially on the precise alignment of the pump and erase beams with the axis of the focusing objective. Here, we propose a new design method for a two-color spiral phase plate with a single-layer structure (S2SPP), and we experimentally investigate the image properties given by the phase plate. In spite of its simple structure, the plate can provide a super-resolution image with a spatial resolution better than 70 nm. Beside eliminating alignment problems and yielding a compact setup, the simplicity of fabrication of the S2SPP makes our proposed method very suitable for commercial microscope systems.

Original languageEnglish
Pages (from-to)353-361
Number of pages9
JournalApplied Spectroscopy
Volume68
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Fluorescence microscopy
Microscopes
microscopy
fluorescence
Optical resolving power
Image resolution
alignment
microscopes
Fluorescence
image resolution
Pumps
Color
Fabrication
depletion
spatial resolution
pumps
color
fabrication

Keywords

  • Fluorescence microscopy
  • Nonlinear microscopy
  • Optical vortices
  • Phase modulation
  • Super-resolution

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

New design method for a phase plate in super-resolution fluorescence microscopy. / Bokor, N.; Iketaki, Yoshinori.

In: Applied Spectroscopy, Vol. 68, No. 3, 03.2014, p. 353-361.

Research output: Contribution to journalArticle

@article{10decb19d4014344bc94624ba17389b3,
title = "New design method for a phase plate in super-resolution fluorescence microscopy",
abstract = "The performance of a super-resolution fluorescence depletion microscope system depends crucially on the precise alignment of the pump and erase beams with the axis of the focusing objective. Here, we propose a new design method for a two-color spiral phase plate with a single-layer structure (S2SPP), and we experimentally investigate the image properties given by the phase plate. In spite of its simple structure, the plate can provide a super-resolution image with a spatial resolution better than 70 nm. Beside eliminating alignment problems and yielding a compact setup, the simplicity of fabrication of the S2SPP makes our proposed method very suitable for commercial microscope systems.",
keywords = "Fluorescence microscopy, Nonlinear microscopy, Optical vortices, Phase modulation, Super-resolution",
author = "N. Bokor and Yoshinori Iketaki",
year = "2014",
month = "3",
doi = "10.1366/13-07249",
language = "English",
volume = "68",
pages = "353--361",
journal = "Applied Spectroscopy",
issn = "0003-7028",
publisher = "Society for Applied Spectroscopy",
number = "3",

}

TY - JOUR

T1 - New design method for a phase plate in super-resolution fluorescence microscopy

AU - Bokor, N.

AU - Iketaki, Yoshinori

PY - 2014/3

Y1 - 2014/3

N2 - The performance of a super-resolution fluorescence depletion microscope system depends crucially on the precise alignment of the pump and erase beams with the axis of the focusing objective. Here, we propose a new design method for a two-color spiral phase plate with a single-layer structure (S2SPP), and we experimentally investigate the image properties given by the phase plate. In spite of its simple structure, the plate can provide a super-resolution image with a spatial resolution better than 70 nm. Beside eliminating alignment problems and yielding a compact setup, the simplicity of fabrication of the S2SPP makes our proposed method very suitable for commercial microscope systems.

AB - The performance of a super-resolution fluorescence depletion microscope system depends crucially on the precise alignment of the pump and erase beams with the axis of the focusing objective. Here, we propose a new design method for a two-color spiral phase plate with a single-layer structure (S2SPP), and we experimentally investigate the image properties given by the phase plate. In spite of its simple structure, the plate can provide a super-resolution image with a spatial resolution better than 70 nm. Beside eliminating alignment problems and yielding a compact setup, the simplicity of fabrication of the S2SPP makes our proposed method very suitable for commercial microscope systems.

KW - Fluorescence microscopy

KW - Nonlinear microscopy

KW - Optical vortices

KW - Phase modulation

KW - Super-resolution

UR - http://www.scopus.com/inward/record.url?scp=84894633274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894633274&partnerID=8YFLogxK

U2 - 10.1366/13-07249

DO - 10.1366/13-07249

M3 - Article

AN - SCOPUS:84894633274

VL - 68

SP - 353

EP - 361

JO - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 0003-7028

IS - 3

ER -