New data on the immunocytochemical localization of thyrotropin-releasing hormone in the rat central nervous system

I. Merchenthaler, V. Csernus, C. Csontos, P. Petrusz, B. Mess

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Abstract

An antiserum raised against the synthetic tripeptide pyroglutamyl-histidyl-proline (free acid) was used to localize thyrotropin-releasing hormone (TRH) in the rat central nervous system (CNS) by immunocytochemistry. The distribution of TRH-immunoreactive structures was similar to that reported earlier; i.e., most of the TRH-containing perikarya were located in the parvicellular part of the hypothalamic paraventricular nucleus, the suprachiasmatic portion of the preoptic nucleus, the dorsomedial nucleus, the lateral basal hypothalamus, and the raphe nuclei. Several new locations for TRH-immunoreactive neurons were also observed, including the glomerular layer of the olfactory bulb, the anterior olfactory nuclei, the diagonal band of Broca, the septal nuclei, the sexually dimorphic nucleus of the preoptic area, the reticular thalamic nucleus, the lateral reticular nucelus of the medulla oblongata, and the central gray matter of the mesencephalon. Immunoreactive fibers were seen in the median eminence, the organum vasculosum of the lamina terminalis, the lateral septal nucleus, the medial habenula, the dorsal and ventral parabrachial nuclei, the nucleus of the solitary tract, around the motor nuclei of the cranial nerves, the dorsal vagal complex, and in the reticular formation of the brainstem. In the spinal cord, no immunoreactive perikarya were observed. Immunoreactive processes were present in the lateral funiculus of the white matter and in laminae V-X in the gray matter. Dense terminal-like structures were seen around spinal motor neurons. The distribution of TRH-immunoreactive structures in the CNS suggests that TRH functions both as a neuroendocrine regulator in the hypothalamus and as a neurotransmitter or neuromodulator throughout the CNS.

Original languageEnglish
Pages (from-to)359-376
Number of pages18
JournalAmerican Journal of Anatomy
Volume181
Issue number4
Publication statusPublished - 1988

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Thyrotropin-Releasing Hormone
Central Nervous System
Septal Nuclei
Preoptic Area
Neurotransmitter Agents
Diagonal Band of Broca
Lateral Hypothalamic Area
Habenula
Lateral Thalamic Nuclei
Mediodorsal Thalamic Nucleus
Medulla Oblongata
Median Eminence
Solitary Nucleus
Raphe Nuclei
Reticular Formation
Cranial Nerves
Paraventricular Hypothalamic Nucleus
Olfactory Bulb
Motor Neurons
Mesencephalon

ASJC Scopus subject areas

  • Anatomy

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New data on the immunocytochemical localization of thyrotropin-releasing hormone in the rat central nervous system. / Merchenthaler, I.; Csernus, V.; Csontos, C.; Petrusz, P.; Mess, B.

In: American Journal of Anatomy, Vol. 181, No. 4, 1988, p. 359-376.

Research output: Contribution to journalArticle

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