New Chalcone derivative inhibits ABCB1 in multidrug resistant T-cell lymphoma and colon adenocarcinoma cells

Martina Čižmáriková, Peter Takáč, Gabriella Spengler, Annamária Kincses, Márta Nové, Mária Vilková, Ján Mojžiš

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aim: Development of new potential drugs to overcome multidrug resistance to chemotherapy is a big challenge for cancer treatment. Attention is also given to the natural compounds and their derivatives. The study aimed at evaluating the impact of a new chalcone derivative (1C) on multidrug resistant cell lines, focusing on P-glycoprotein (P-gp, ABCB1) inhibition, as well as 1C-doxorubicin interaction in vitro. Materials and Methods: Cytotoxic and antiproliferative effects of the 1C compound were assessed by thiazolyl blue tetrazolium bromide (MTT) method in mouse T-cell lymphoma and human colon adenocarcinoma cells expressing ABCB1. Alterations in ABCB1 activity were evaluated by rhodamine 123 accumulation assay using flow cytometry. Drug-drug interaction was studied using combination assay. Results: Our results confirmed antiproliferative, cytotoxic, as well as ABCB1 inhibitory potential of 1C in both tested ABCB1-expressing cancer cell lines. Furthermore, 1C displayed synergistic interaction with doxorubicin. Conclusion: Our results suggest the 1C chalcone derivative as a promising compound against resistant lymphoma and colon cancer, which could be used in monotherapy or in combination with other chemotherapeutics.

Original languageEnglish
Pages (from-to)6499-6505
Number of pages7
JournalAnticancer research
Volume39
Issue number12
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • ABCB1
  • Chalcone
  • Colon adenocarcinoma
  • Doxorubicin
  • P-glycoprotein
  • T-lymphoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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