New aspects of the simultaneous analysis of amino acids and amines as their o-phthaldialdehyde derivatives by high-performance liquid chromatography: Analysis of wine, beer and vinegar

D. Kutlán, I. Molnár-Perl

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

A new high-performance liquid chromatography method is described for the simultaneous quantitation of amino acids and amines for 37 compounds (20 amino acids+17 amines), as their o-phthaldialdehyde (OPA)-3-mercaptopropionic acid derivatives, within 53 min. Based on previously documented stoichiometric and reaction mechanism studies, derivatizations have been carried out with the OPA-SH-group=1:50 containing reagents. Reliability and reproducibility of analyses have been considerably improved. Average reproducibility data in a wide concentration range of derivatives had RSD≤3.4%.

Original languageEnglish
Pages (from-to)311-322
Number of pages12
JournalJournal of Chromatography A
Volume987
Issue number1-2
DOIs
Publication statusPublished - Feb 14 2003

Fingerprint

o-Phthalaldehyde
Beer
Wine
High performance liquid chromatography
Acetic Acid
Amines
3-Mercaptopropionic Acid
High Pressure Liquid Chromatography
Derivatives
Amino Acids
compound 20

Keywords

  • Amines
  • Amino acids
  • Beer
  • Derivatization, LC
  • Food analysis
  • Wine

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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abstract = "A new high-performance liquid chromatography method is described for the simultaneous quantitation of amino acids and amines for 37 compounds (20 amino acids+17 amines), as their o-phthaldialdehyde (OPA)-3-mercaptopropionic acid derivatives, within 53 min. Based on previously documented stoichiometric and reaction mechanism studies, derivatizations have been carried out with the OPA-SH-group=1:50 containing reagents. Reliability and reproducibility of analyses have been considerably improved. Average reproducibility data in a wide concentration range of derivatives had RSD≤3.4{\%}.",
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