New approach to analyze genetic and clinical data in bisphosphonate-induced osteonecrosis of the jaw

B. Balla, M. Vaszilko, J. Kósa, J. Podaní, I. Takács, B. Tóbiás, Z. Nagy, Á Lazáry, P. Lakatos

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objectives: Osteonecrosis of the jaw (ONJ) is a major complication associated with long-term use of bisphosphonates (BP). We aimed to investigate the effect of CYP2C8 rs1934951 SNP and its relationship to a number of clinical and biochemical factors in 46 Hungarian subjects with bisphosphonate-induced ONJ. Methods: Blood samples were collected from each subject and genomic DNA was extracted. SNP analysis of CYP2C8 gene was carried out by predesigned TaqMan primer/probe sets. The genetic data together with clinical and biochemical variables were evaluated by chi-square test, logistic regression, and principal component analysis (PCA). Results: The risk of mandibular localization of ONJ was 19.2-fold higher in subjects with AG genotype than in normal GG genotype. PCA revealed strong positive correlations between maxillar localization of ONJ and a group of variables including intravenous BP application and serum lipid markers. Mandibular localization of ONJ was correlated positively with serum calcium, 25-hydroxy-vitamin D and PTH levels, oral BP application, and the length of BP therapy. The degree of the disease and the number of recurrences were correlated with the application of hormone-deprivation therapy for breast cancer patients. Conclusion: The statistical approach applying PCA to our data may contribute to the better understanding of factors playing role in the development of bisphosphonate-induced ONJ.

Original languageEnglish
Pages (from-to)580-585
Number of pages6
JournalOral Diseases
Volume18
Issue number6
DOIs
Publication statusPublished - Sep 2012

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Bisphosphonate-Associated Osteonecrosis of the Jaw
Osteonecrosis
Diphosphonates
Jaw
Principal Component Analysis
Single Nucleotide Polymorphism
Genotype
Chi-Square Distribution
Vitamin D
Biomarkers
Logistic Models
Hormones
Breast Neoplasms
Calcium
Lipids
Recurrence
DNA
Therapeutics
Serum
Genes

Keywords

  • CYP2C8 polymorphism
  • Mandible
  • Maxilla
  • Osteonecrosis of the jaws
  • Principal component analysis

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology

Cite this

New approach to analyze genetic and clinical data in bisphosphonate-induced osteonecrosis of the jaw. / Balla, B.; Vaszilko, M.; Kósa, J.; Podaní, J.; Takács, I.; Tóbiás, B.; Nagy, Z.; Lazáry, Á; Lakatos, P.

In: Oral Diseases, Vol. 18, No. 6, 09.2012, p. 580-585.

Research output: Contribution to journalArticle

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