New adenovirus species found in a patient presenting with gastroenteritis

Morris Saffold Jones, B. Harrach, Robert D. Ganac, Mary M A Gozum, Wilfred P. Dela Cruz, Brian Riedel, Chao Pan, Eric L. Delwart, David P. Schnurr

Research output: Contribution to journalArticle

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Abstract

An unidentified agent was cultured in primary monkey cells at the Los Angeles County Public Health Department from each of five stool specimens submitted from an outbreak of gastroenteritis. Electron microscopy and an adenovirus-specific monoclonal antibody confirmed this agent to be an adenovirus. Since viral titers were too low, complete serotyping was not possible. Using the DNase-sequence-independent viral nucleic acid amplification method, we identified several nucleotide sequences with a high homology to human adenovirus 41 (HAdV-41) and simian adenovirus 1 (SAdV-1). However, using anti-SAdV-1 sera, it was determined that this virus was serologically different than SAdV-1. Genomic sequencing and phylogenetic analysis confirmed that this new adenovirus was so divergent from the known human adenoviruses that it was not only a new type but also represented a new species (human adenovirus G). In a retrospective clinical study, this new virus was detected by PCR in one additional patient from a separate gastroenteritis outbreak. This study suggests that HAdV-52 may be one of many agents causing gastroenteritis of unknown etiology.

Original languageEnglish
Pages (from-to)5978-5984
Number of pages7
JournalJournal of Virology
Volume81
Issue number11
DOIs
Publication statusPublished - Jun 2007

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Human adenovirus G
Simian Adenoviruses
Human Adenoviruses
gastroenteritis
Gastroenteritis
Adenoviridae
Disease Outbreaks
Human adenovirus F
Viruses
Serotyping
Deoxyribonucleases
Los Angeles
viruses
deoxyribonucleases
Nucleic Acids
Haplorhini
Electron Microscopy
Retrospective Studies
Public Health
viral load

ASJC Scopus subject areas

  • Immunology

Cite this

Jones, M. S., Harrach, B., Ganac, R. D., Gozum, M. M. A., Dela Cruz, W. P., Riedel, B., ... Schnurr, D. P. (2007). New adenovirus species found in a patient presenting with gastroenteritis. Journal of Virology, 81(11), 5978-5984. https://doi.org/10.1128/JVI.02650-06

New adenovirus species found in a patient presenting with gastroenteritis. / Jones, Morris Saffold; Harrach, B.; Ganac, Robert D.; Gozum, Mary M A; Dela Cruz, Wilfred P.; Riedel, Brian; Pan, Chao; Delwart, Eric L.; Schnurr, David P.

In: Journal of Virology, Vol. 81, No. 11, 06.2007, p. 5978-5984.

Research output: Contribution to journalArticle

Jones, MS, Harrach, B, Ganac, RD, Gozum, MMA, Dela Cruz, WP, Riedel, B, Pan, C, Delwart, EL & Schnurr, DP 2007, 'New adenovirus species found in a patient presenting with gastroenteritis', Journal of Virology, vol. 81, no. 11, pp. 5978-5984. https://doi.org/10.1128/JVI.02650-06
Jones, Morris Saffold ; Harrach, B. ; Ganac, Robert D. ; Gozum, Mary M A ; Dela Cruz, Wilfred P. ; Riedel, Brian ; Pan, Chao ; Delwart, Eric L. ; Schnurr, David P. / New adenovirus species found in a patient presenting with gastroenteritis. In: Journal of Virology. 2007 ; Vol. 81, No. 11. pp. 5978-5984.
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