Neutron scattering studies on dUTPase complex in the presence of bioprotectant systems

B. Varga, F. Migliardo, E. Takacs, B. Vértessy, S. Magazù, C. Mondelli

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The aim of the present work is to investigate the chemical physics mechanisms of protein stabilization by homologous disaccharides (trehalose, maltose and sucrose). On this purpose the structural and dynamical properties of dUTPase-inhibitor candidate and dUTPase-inhibitor candidate/disaccharide mixtures have been investigated by elastic neutron scattering (ENS), quasi-elastic neutron scattering (QENS) and photon correlation spectroscopy (PCS). The decrease in the ENS intensity profiles vs temperature for the disaccharide-water mixtures is less marked in the case of trehalose/water mixture. This indicates that trehalose shows a larger structural resistance to temperature changes and a higher "rigidity" in comparison with maltose/H2O and sucrose/H2O mixtures. In addition the protein/hydrated-disaccharide mixtures show a linear dependence between the solvent viscosity and the local mean-square displacement of hydrated dUTpase/disaccharide systems. This result shows that the protein dynamics is coupled with that of the surrounding matrix. Furthermore, QENS results on the binary disaccharide-H2O/D2O mixtures indicate that the water dynamics is affected by all the disaccharides and particularly by trehalose. Finally, PCS findings indicate that the protein hydrodynamic radius in solution does not change at low disaccharide concentrations, while reveal, at high disaccharide concentration, a breakdown of the Stokes-Einstein law. The experimental findings are discussed and interpreted in the frame of the current theories.

Original languageEnglish
Pages (from-to)250-258
Number of pages9
JournalChemical Physics
Volume345
Issue number2-3
DOIs
Publication statusPublished - Apr 18 2008

Fingerprint

Disaccharides
Neutron scattering
neutron scattering
elastic scattering
Trehalose
Elastic scattering
proteins
sucrose
inhibitors
Photon correlation spectroscopy
Maltose
water
Sucrose
Water
photons
Proteins
rigidity
temperature profiles
spectroscopy
dUTP pyrophosphatase

Keywords

  • Disaccharides
  • Dynamical properties
  • Neutron scattering
  • Protein
  • Stabilization
  • Structural properties

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

Neutron scattering studies on dUTPase complex in the presence of bioprotectant systems. / Varga, B.; Migliardo, F.; Takacs, E.; Vértessy, B.; Magazù, S.; Mondelli, C.

In: Chemical Physics, Vol. 345, No. 2-3, 18.04.2008, p. 250-258.

Research output: Contribution to journalArticle

Varga, B. ; Migliardo, F. ; Takacs, E. ; Vértessy, B. ; Magazù, S. ; Mondelli, C. / Neutron scattering studies on dUTPase complex in the presence of bioprotectant systems. In: Chemical Physics. 2008 ; Vol. 345, No. 2-3. pp. 250-258.
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