Neutralizing and enhancing antibodies measured in complement-restored serum samples from HIV-1-infected individuals correlate with immunosuppression and disease

G. Füst, F. Tóth, Jolán Kiss, E. Újhelyi, Imre Nagy, D. Bánhegyi

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Abstract

Objective: To study the association between the progression of HIV disease and HIV neutralization and enhancement measured in the presence of human complement. Design: Two studies were performed: (1) longitudinal measurement of the complement-dependent enhancing antibodies in parallel with T-cell subset determination in 55 serum samples from seven HIV-infected patients, and (2) determination of the titres of neutralizing and enhancing antibodies in stored samples of 21 HIV-asymptomatic patients obtained between 1986 and 1987 and follow-up of the patients until October 1992. Methods: HIV-1 [human T-lymphotropic virus (HTLV)(IIIB) strain, 100 median tissue culture infective dose (TCID50)] was incubated with twofold dilutions of sera in the presence of human complement (final dilution, 1:4) and added to MT-4 cells. HIV growth was monitored daily for 5 days using the reclustering inhibition and p24 immunofluorescence assays. Results: A significant negative correlation between the titres of enhancing antibodies and CD4+ cell count was found in longitudinal measurements. In the prospective studies, marked differences were observed between patients with undetectable, low, or high titres of enhancing antibodies in the clinical course of HIV disease: CD4+ cell counts and percentages decreased more rapidly in the high titre group within 3 years. After 5 years, AIDS developed in five out of six patients in the high titre group but only in five out of 15 of the low titre group (P <0.05). A similar difference was observed between patients with and without neutralizing antibodies. Conclusions: Measurement of HIV neutralization and enhancement in complement-containing serum samples using a complement receptor carrying target may provide data of clinical relevance. Neutralization appears to be associated with a favourable prognosis whereas high titre enhancing antibodies predict rapid progression of HIV disease.

Original languageEnglish
Pages (from-to)603-609
Number of pages7
JournalAIDS
Volume8
Issue number5
Publication statusPublished - May 1994

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Blocking Antibodies
Neutralizing Antibodies
Immunosuppression
HIV-1
HIV
Serum
CD4 Lymphocyte Count
Disease Progression
Complement Receptors
T-Lymphocyte Subsets
Fluorescent Antibody Technique
Acquired Immunodeficiency Syndrome
Prospective Studies
Viruses
Growth

Keywords

  • AIDS
  • Antibody
  • Complement
  • HIV
  • Infection enhancement
  • Neutralization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

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title = "Neutralizing and enhancing antibodies measured in complement-restored serum samples from HIV-1-infected individuals correlate with immunosuppression and disease",
abstract = "Objective: To study the association between the progression of HIV disease and HIV neutralization and enhancement measured in the presence of human complement. Design: Two studies were performed: (1) longitudinal measurement of the complement-dependent enhancing antibodies in parallel with T-cell subset determination in 55 serum samples from seven HIV-infected patients, and (2) determination of the titres of neutralizing and enhancing antibodies in stored samples of 21 HIV-asymptomatic patients obtained between 1986 and 1987 and follow-up of the patients until October 1992. Methods: HIV-1 [human T-lymphotropic virus (HTLV)(IIIB) strain, 100 median tissue culture infective dose (TCID50)] was incubated with twofold dilutions of sera in the presence of human complement (final dilution, 1:4) and added to MT-4 cells. HIV growth was monitored daily for 5 days using the reclustering inhibition and p24 immunofluorescence assays. Results: A significant negative correlation between the titres of enhancing antibodies and CD4+ cell count was found in longitudinal measurements. In the prospective studies, marked differences were observed between patients with undetectable, low, or high titres of enhancing antibodies in the clinical course of HIV disease: CD4+ cell counts and percentages decreased more rapidly in the high titre group within 3 years. After 5 years, AIDS developed in five out of six patients in the high titre group but only in five out of 15 of the low titre group (P <0.05). A similar difference was observed between patients with and without neutralizing antibodies. Conclusions: Measurement of HIV neutralization and enhancement in complement-containing serum samples using a complement receptor carrying target may provide data of clinical relevance. Neutralization appears to be associated with a favourable prognosis whereas high titre enhancing antibodies predict rapid progression of HIV disease.",
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author = "G. F{\"u}st and F. T{\'o}th and Jol{\'a}n Kiss and E. {\'U}jhelyi and Imre Nagy and D. B{\'a}nhegyi",
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T1 - Neutralizing and enhancing antibodies measured in complement-restored serum samples from HIV-1-infected individuals correlate with immunosuppression and disease

AU - Füst, G.

AU - Tóth, F.

AU - Kiss, Jolán

AU - Újhelyi, E.

AU - Nagy, Imre

AU - Bánhegyi, D.

PY - 1994/5

Y1 - 1994/5

N2 - Objective: To study the association between the progression of HIV disease and HIV neutralization and enhancement measured in the presence of human complement. Design: Two studies were performed: (1) longitudinal measurement of the complement-dependent enhancing antibodies in parallel with T-cell subset determination in 55 serum samples from seven HIV-infected patients, and (2) determination of the titres of neutralizing and enhancing antibodies in stored samples of 21 HIV-asymptomatic patients obtained between 1986 and 1987 and follow-up of the patients until October 1992. Methods: HIV-1 [human T-lymphotropic virus (HTLV)(IIIB) strain, 100 median tissue culture infective dose (TCID50)] was incubated with twofold dilutions of sera in the presence of human complement (final dilution, 1:4) and added to MT-4 cells. HIV growth was monitored daily for 5 days using the reclustering inhibition and p24 immunofluorescence assays. Results: A significant negative correlation between the titres of enhancing antibodies and CD4+ cell count was found in longitudinal measurements. In the prospective studies, marked differences were observed between patients with undetectable, low, or high titres of enhancing antibodies in the clinical course of HIV disease: CD4+ cell counts and percentages decreased more rapidly in the high titre group within 3 years. After 5 years, AIDS developed in five out of six patients in the high titre group but only in five out of 15 of the low titre group (P <0.05). A similar difference was observed between patients with and without neutralizing antibodies. Conclusions: Measurement of HIV neutralization and enhancement in complement-containing serum samples using a complement receptor carrying target may provide data of clinical relevance. Neutralization appears to be associated with a favourable prognosis whereas high titre enhancing antibodies predict rapid progression of HIV disease.

AB - Objective: To study the association between the progression of HIV disease and HIV neutralization and enhancement measured in the presence of human complement. Design: Two studies were performed: (1) longitudinal measurement of the complement-dependent enhancing antibodies in parallel with T-cell subset determination in 55 serum samples from seven HIV-infected patients, and (2) determination of the titres of neutralizing and enhancing antibodies in stored samples of 21 HIV-asymptomatic patients obtained between 1986 and 1987 and follow-up of the patients until October 1992. Methods: HIV-1 [human T-lymphotropic virus (HTLV)(IIIB) strain, 100 median tissue culture infective dose (TCID50)] was incubated with twofold dilutions of sera in the presence of human complement (final dilution, 1:4) and added to MT-4 cells. HIV growth was monitored daily for 5 days using the reclustering inhibition and p24 immunofluorescence assays. Results: A significant negative correlation between the titres of enhancing antibodies and CD4+ cell count was found in longitudinal measurements. In the prospective studies, marked differences were observed between patients with undetectable, low, or high titres of enhancing antibodies in the clinical course of HIV disease: CD4+ cell counts and percentages decreased more rapidly in the high titre group within 3 years. After 5 years, AIDS developed in five out of six patients in the high titre group but only in five out of 15 of the low titre group (P <0.05). A similar difference was observed between patients with and without neutralizing antibodies. Conclusions: Measurement of HIV neutralization and enhancement in complement-containing serum samples using a complement receptor carrying target may provide data of clinical relevance. Neutralization appears to be associated with a favourable prognosis whereas high titre enhancing antibodies predict rapid progression of HIV disease.

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