Neutralization of Neisseria meningitidis outer membrane vesicles

András Vida, Annet Troelstra, P. Antal-Szalmás, Toon J P Van Bommel, André F M Verheul, Jan Verhoef, Kok P M Van Kessel, Jos A G Van Strijp

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: We aimed to determine the neutralization of Neisseria meningitidis outer membrane vesicles (blebs) by humoral and cellular elements of whole blood. Methods: The interaction of FITC-labeled blebs with monocytes was studied by spectrofluorometry. Blebs are able to induce an oxidative burst in neutrophils, and we evaluated the inhibitory effect of plasma on this process. Results: Human plasma reduced the priming activity of blebs containing 1-3 ng/ml lipopolysaccharide (LPS) by 50-60% and bactericidal permeability increasing protein (BPI) reduced priming to background levels. A complete neutralization of LPS and blebs by plasma and BPI was measured using the limulus amebocyte lysate (LAL) assay. Furthermore, only 3% of blebs were cell-associated, while the remainder were in the supernatant. Conclusions: Plasma and BPI are able to neutralize blebs, with phagocytosis playing only a minor role. As such, we conclude that blebs do not behave like particles but more like free LPS.

Original languageEnglish
Pages (from-to)801-805
Number of pages5
JournalInflammation Research
Volume60
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Neisseria meningitidis
Blister
Membranes
Lipopolysaccharides
Horseshoe Crabs
Respiratory Burst
Fluorescein-5-isothiocyanate
Fluorescence Spectrometry
Phagocytosis
Monocytes
Neutrophils

Keywords

  • BPI
  • CD14
  • LPS
  • Neisseria meningitidis
  • Outer membrane vesicles

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

Vida, A., Troelstra, A., Antal-Szalmás, P., Van Bommel, T. J. P., Verheul, A. F. M., Verhoef, J., ... Van Strijp, J. A. G. (2011). Neutralization of Neisseria meningitidis outer membrane vesicles. Inflammation Research, 60(9), 801-805. https://doi.org/10.1007/s00011-011-0344-3

Neutralization of Neisseria meningitidis outer membrane vesicles. / Vida, András; Troelstra, Annet; Antal-Szalmás, P.; Van Bommel, Toon J P; Verheul, André F M; Verhoef, Jan; Van Kessel, Kok P M; Van Strijp, Jos A G.

In: Inflammation Research, Vol. 60, No. 9, 09.2011, p. 801-805.

Research output: Contribution to journalArticle

Vida, A, Troelstra, A, Antal-Szalmás, P, Van Bommel, TJP, Verheul, AFM, Verhoef, J, Van Kessel, KPM & Van Strijp, JAG 2011, 'Neutralization of Neisseria meningitidis outer membrane vesicles', Inflammation Research, vol. 60, no. 9, pp. 801-805. https://doi.org/10.1007/s00011-011-0344-3
Vida, András ; Troelstra, Annet ; Antal-Szalmás, P. ; Van Bommel, Toon J P ; Verheul, André F M ; Verhoef, Jan ; Van Kessel, Kok P M ; Van Strijp, Jos A G. / Neutralization of Neisseria meningitidis outer membrane vesicles. In: Inflammation Research. 2011 ; Vol. 60, No. 9. pp. 801-805.
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