Neurotrophic effect of hematopoietic cytokines on cholinergic and other neurons in vitro

T. Tabira, Y. Konishi, F. Gallyas

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We examined the effects of interleukin-3 (IL-3) and other hematopoietic cytokines on the neurotransmitters, neurite formation, and differentiation in cholinergic and other types of neurons. IL-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor, granulocyte colony-stimulating factor and erythropoietin (Epo) elevated choline acetyltransferase (ChAT) activity in septal cholinergic cell line SN6 as well as in primary cultured septal neurons without increasing protein contents of the cells. These effects were dose-dependent and the optimal doses were not different from those for blood cells. IL-3 had neurite-promoting activity but GM-CSF had no such effect. Both IL-3 and GM-CSF decreased intracellular acetylcholine concentration, and elevated glutamic acid decarboxylase and intracellular GABA in septal neuronal cultures. Epo elevated monoamines in PC12 cells. These effects are thought to result from direct action through their specific receptors in neurons, because (i) anti-IL-3-receptor antibody abolished the ChAT activity in septal neurons increased by IL-3; (ii) mRNA and immunoreactivity for β subunits of IL-3 receptors were expressed in septal cholinergic neurons and (iii) presence of receptos for GM-CSF and Epo in neurons has been reported. Our observation and others strongly support that neural-immune interactions are important not only in the defense mechanism in the nervous system but also in the development, differentiation and function of neurons.

Original languageEnglish
Pages (from-to)241-252
Number of pages12
JournalInternational Journal of Developmental Neuroscience
Volume13
Issue number3-4
DOIs
Publication statusPublished - 1995

Fingerprint

Cholinergic Neurons
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Neurons
Interleukin-3 Receptors
Erythropoietin
Choline O-Acetyltransferase
Neurites
Glutamate Decarboxylase
Macrophage Colony-Stimulating Factor
PC12 Cells
Granulocyte Colony-Stimulating Factor
gamma-Aminobutyric Acid
Cholinergic Agents
Nervous System
Acetylcholine
Neurotransmitter Agents
Blood Cells
Observation

Keywords

  • hematopoietic cytokines
  • immune system
  • nervous system
  • neurotransmitters

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Neurotrophic effect of hematopoietic cytokines on cholinergic and other neurons in vitro. / Tabira, T.; Konishi, Y.; Gallyas, F.

In: International Journal of Developmental Neuroscience, Vol. 13, No. 3-4, 1995, p. 241-252.

Research output: Contribution to journalArticle

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