Neurotransmitter systems of commissural interneurons in the lumbar spinal cord of neonatal rats

Ildikó Wéber, Gábor Veress, Péter Szucs, M. Antal, András Birinyi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The circuits that generate rhythmic locomotor activities are located in the ventromedial area of the lumbar spinal cord and comprise commissural interneurons necessary for left-right alternation during walking movements. In this study we injected biotinylated dextran amine (BDA) into the ventromedial gray matter of the lumbar spinal cord of neonatal rats to label commissural interneurons. Anterogradely labeled axons arose from the site of injection, crossed the midline in the anterior commissure and arborized extensively in the contralateral ventral horn of the spinal cord. The presence of neurotransmitter systems in labeled axon terminals of commissural interneurons was investigated by using antibodies raised against specific transmitter-related proteins. Boutons potentially containing inhibitory amino acids were identified by applying glutamic acid decarboxylase (GAD65/67) and glycine transporter 2 antibodies. Out of 1146 BDA-labeled axon terminals, 663 boutons were assumed on this basis to be inhibitory; 76% of these terminals were immunoreactive for glycine transporter, 53% were immunoreactive for GAD and about 30% of inhibitory boutons might contain both inhibitory amino acids. Boutons potentially containing putative excitatory neurotransmitter were revealed with antibodies raised against vesicular glutamate transporters 1 and 2. Out of 590 BDA-labeled boutons about one fourth (158) were immunoreactive for glutamate transporters. These mammalian commissural interneurons are compared to the glycinergic commissural interneurons in the swimming CPGs of lamprey and the Xenopus tadpole. Our results show that commissural interneurons in the mammalian spinal cord form a heterogeneous group including glutamatergic excitatory and GABAergic and glycinergic inhibitory neurons.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalBrain Research
Volume1178
Issue number1
DOIs
Publication statusPublished - Oct 31 2007

Fingerprint

Neurotransmitter Agents
Spinal Cord
Glycine Plasma Membrane Transport Proteins
Presynaptic Terminals
Antibodies
Vesicular Glutamate Transport Protein 1
Vesicular Glutamate Transport Protein 2
Lampreys
Amino Acid Transport System X-AG
Amino Acids
Glutamate Decarboxylase
Locomotion
Xenopus
Walking
Larva
Axons
Commissural Interneurons
Neurons
Injections
biotinylated dextran amine

Keywords

  • GABA
  • Glutamate
  • Glycine
  • Locomotion
  • Neural tracing

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Neurotransmitter systems of commissural interneurons in the lumbar spinal cord of neonatal rats. / Wéber, Ildikó; Veress, Gábor; Szucs, Péter; Antal, M.; Birinyi, András.

In: Brain Research, Vol. 1178, No. 1, 31.10.2007, p. 65-72.

Research output: Contribution to journalArticle

Wéber, Ildikó ; Veress, Gábor ; Szucs, Péter ; Antal, M. ; Birinyi, András. / Neurotransmitter systems of commissural interneurons in the lumbar spinal cord of neonatal rats. In: Brain Research. 2007 ; Vol. 1178, No. 1. pp. 65-72.
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