Neurotensin in the rat median eminence: the possible sources of neurotensin-like fibers and varicosities in the external layer

Alexander Kiss, M. Palkóvits, Ferenc A. Antoni, Robert L. Eskay, Lana R. Skirboll

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The possible sources of neurotensin-like immunoreactive axons in the median eminence were studied after several experimental surgical approaches including unilateral lateral retrochiasmatic area transection, midsagittal knife cut through the median eminence, complete surgical isolation of the medial basal hypothalamus and bilateral paraventricular nucleus lesions. Both immunohistochemical and radioimmunoassay data demonstrate that neurotensin-containing neuronal located in the hypothalamic arcuate nuclei represent the main source of neurotensin occurring in the external zone of the median eminence of the rat: (1) neither the complete isolation of the medial basal hypothalamus nor the transection of the major neuronal input channel to the median eminence in the lateral retrochiasmatic area altered neurotensin-like immunoreactivity in the median eminence; (2) bilateral lesioning of the paraventricular nucleus resulted in insignificant changes of neurotensin level in the median eminence; and (3) two days after lesioning the median eminence an increased amount of retrogradely accumulated neurotensin-like immunoreactivity was found in several perikarya of the arcuate nuclei due to the blockage of axonal transport in the transected fibers. Retrograde accumulation of neurotensin-like material in other cells scattered in the anterior hypothalamus (in the paraventricular, paraventricular and anterior hypothalamic nuclei) indicates that in addition to the arcuate neurons these neurons may also participate in the neurotensin innervation of the median eminence.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalBrain Research
Volume416
Issue number1
DOIs
Publication statusPublished - Jul 21 1987

Fingerprint

Neurotensin
Median Eminence
Paraventricular Hypothalamic Nucleus
Middle Hypothalamus
Arcuate Nucleus of Hypothalamus
Anterior Hypothalamic Nucleus
Anterior Hypothalamus
Neurons
Axonal Transport
Radioimmunoassay
Axons

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neurotensin in the rat median eminence : the possible sources of neurotensin-like fibers and varicosities in the external layer. / Kiss, Alexander; Palkóvits, M.; Antoni, Ferenc A.; Eskay, Robert L.; Skirboll, Lana R.

In: Brain Research, Vol. 416, No. 1, 21.07.1987, p. 129-135.

Research output: Contribution to journalArticle

Kiss, Alexander ; Palkóvits, M. ; Antoni, Ferenc A. ; Eskay, Robert L. ; Skirboll, Lana R. / Neurotensin in the rat median eminence : the possible sources of neurotensin-like fibers and varicosities in the external layer. In: Brain Research. 1987 ; Vol. 416, No. 1. pp. 129-135.
@article{b2e283590213413aad8653f3ad31bac1,
title = "Neurotensin in the rat median eminence: the possible sources of neurotensin-like fibers and varicosities in the external layer",
abstract = "The possible sources of neurotensin-like immunoreactive axons in the median eminence were studied after several experimental surgical approaches including unilateral lateral retrochiasmatic area transection, midsagittal knife cut through the median eminence, complete surgical isolation of the medial basal hypothalamus and bilateral paraventricular nucleus lesions. Both immunohistochemical and radioimmunoassay data demonstrate that neurotensin-containing neuronal located in the hypothalamic arcuate nuclei represent the main source of neurotensin occurring in the external zone of the median eminence of the rat: (1) neither the complete isolation of the medial basal hypothalamus nor the transection of the major neuronal input channel to the median eminence in the lateral retrochiasmatic area altered neurotensin-like immunoreactivity in the median eminence; (2) bilateral lesioning of the paraventricular nucleus resulted in insignificant changes of neurotensin level in the median eminence; and (3) two days after lesioning the median eminence an increased amount of retrogradely accumulated neurotensin-like immunoreactivity was found in several perikarya of the arcuate nuclei due to the blockage of axonal transport in the transected fibers. Retrograde accumulation of neurotensin-like material in other cells scattered in the anterior hypothalamus (in the paraventricular, paraventricular and anterior hypothalamic nuclei) indicates that in addition to the arcuate neurons these neurons may also participate in the neurotensin innervation of the median eminence.",
author = "Alexander Kiss and M. Palk{\'o}vits and Antoni, {Ferenc A.} and Eskay, {Robert L.} and Skirboll, {Lana R.}",
year = "1987",
month = "7",
day = "21",
doi = "10.1016/0006-8993(87)91504-6",
language = "English",
volume = "416",
pages = "129--135",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Neurotensin in the rat median eminence

T2 - the possible sources of neurotensin-like fibers and varicosities in the external layer

AU - Kiss, Alexander

AU - Palkóvits, M.

AU - Antoni, Ferenc A.

AU - Eskay, Robert L.

AU - Skirboll, Lana R.

PY - 1987/7/21

Y1 - 1987/7/21

N2 - The possible sources of neurotensin-like immunoreactive axons in the median eminence were studied after several experimental surgical approaches including unilateral lateral retrochiasmatic area transection, midsagittal knife cut through the median eminence, complete surgical isolation of the medial basal hypothalamus and bilateral paraventricular nucleus lesions. Both immunohistochemical and radioimmunoassay data demonstrate that neurotensin-containing neuronal located in the hypothalamic arcuate nuclei represent the main source of neurotensin occurring in the external zone of the median eminence of the rat: (1) neither the complete isolation of the medial basal hypothalamus nor the transection of the major neuronal input channel to the median eminence in the lateral retrochiasmatic area altered neurotensin-like immunoreactivity in the median eminence; (2) bilateral lesioning of the paraventricular nucleus resulted in insignificant changes of neurotensin level in the median eminence; and (3) two days after lesioning the median eminence an increased amount of retrogradely accumulated neurotensin-like immunoreactivity was found in several perikarya of the arcuate nuclei due to the blockage of axonal transport in the transected fibers. Retrograde accumulation of neurotensin-like material in other cells scattered in the anterior hypothalamus (in the paraventricular, paraventricular and anterior hypothalamic nuclei) indicates that in addition to the arcuate neurons these neurons may also participate in the neurotensin innervation of the median eminence.

AB - The possible sources of neurotensin-like immunoreactive axons in the median eminence were studied after several experimental surgical approaches including unilateral lateral retrochiasmatic area transection, midsagittal knife cut through the median eminence, complete surgical isolation of the medial basal hypothalamus and bilateral paraventricular nucleus lesions. Both immunohistochemical and radioimmunoassay data demonstrate that neurotensin-containing neuronal located in the hypothalamic arcuate nuclei represent the main source of neurotensin occurring in the external zone of the median eminence of the rat: (1) neither the complete isolation of the medial basal hypothalamus nor the transection of the major neuronal input channel to the median eminence in the lateral retrochiasmatic area altered neurotensin-like immunoreactivity in the median eminence; (2) bilateral lesioning of the paraventricular nucleus resulted in insignificant changes of neurotensin level in the median eminence; and (3) two days after lesioning the median eminence an increased amount of retrogradely accumulated neurotensin-like immunoreactivity was found in several perikarya of the arcuate nuclei due to the blockage of axonal transport in the transected fibers. Retrograde accumulation of neurotensin-like material in other cells scattered in the anterior hypothalamus (in the paraventricular, paraventricular and anterior hypothalamic nuclei) indicates that in addition to the arcuate neurons these neurons may also participate in the neurotensin innervation of the median eminence.

UR - http://www.scopus.com/inward/record.url?scp=0023185392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023185392&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(87)91504-6

DO - 10.1016/0006-8993(87)91504-6

M3 - Article

C2 - 3304533

AN - SCOPUS:0023185392

VL - 416

SP - 129

EP - 135

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -