Neuropeptide S-and Neuropeptide S receptor-expressing neuron populations in the human pons

C. Ádori, Swapnali Barde, Nenad Bogdanovic, Mathias Uhlén, Rainer R. Reinscheid, Gabor G. Kovacs, Tomas Hökfelt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Neuropeptide S (NPS) is a regulatory peptide with potent pharmacological effects. In rodents, NPS is expressed in a few pontine cell clusters. Its receptor (NPSR1) is, however, widely distributed in the brain. The anxiolytic and arousal-promoting effects of NPS make the NPS-NPSR1 system an interesting potential drug target in mood-related disorders. However, so far possible disease-related mechanisms involving NPS have only been studied in rodents. To validate the relevance of these animal studies for i.a. drug development, we have explored the distribution of NPS-expressing neurons in the human pons using in situ hybridization and stereological methods and we compared the distribution of NPS mRNA expressing neurons in the human and rat brain. The calculation revealed a total number of 22,317 ± 2411 NPS mRNA-positive neurons in human, bilaterally. The majority of cells (84%) were located in the parabrachial area in human: in the extension of the medial and lateral parabrachial nuclei, in the Kölliker-Fuse nucleus and around the adjacent lateral lemniscus. In human, in sharp contrast to the rodents, only very few NPS-positive cells (5%) were found close to the locus coeruleus. In addition, we identified a smaller cell cluster (11% of all NPS cells) in the pontine central gray matter both in human and rat, which has not been described previously even in rodents. We also examined the distribution of NPSR1 mRNA-expressing neurons in the human pons. These cells were mainly located in the rostral laterodorsal tegmental nucleus, the cuneiform nucleus, the microcellular tegmental nucleus region and in the periaqueductal gray. Our results show that both NPS and NPSR1 in the human pons are preferentially localized in regions of importance for integration of visceral autonomic information and emotional behavior. The reported interspecies differences must, however, be considered when looking for targets for new pharmacotherapeutical interventions.

Original languageEnglish
Article numberA126
JournalFrontiers in Neuroanatomy
Volume9
Issue numberSeptember
DOIs
Publication statusPublished - Sep 25 2015

Fingerprint

Neuropeptide Receptors
Pons
Neuropeptides
Neurons
Population
Rodentia
Messenger RNA
Periaqueductal Gray
Locus Coeruleus
Anti-Anxiety Agents
Brain
Arousal
Mood Disorders
Pharmaceutical Preparations
In Situ Hybridization

Keywords

  • Anxiety
  • Arousal
  • Deep brain stimulation (DBS)
  • Human brain
  • Neuropeptide S (NPS)
  • Parabrachial
  • Periaqueductal gray
  • Sudden infant death syndrome

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience (miscellaneous)
  • Cellular and Molecular Neuroscience

Cite this

Ádori, C., Barde, S., Bogdanovic, N., Uhlén, M., Reinscheid, R. R., Kovacs, G. G., & Hökfelt, T. (2015). Neuropeptide S-and Neuropeptide S receptor-expressing neuron populations in the human pons. Frontiers in Neuroanatomy, 9(September), [A126]. https://doi.org/10.3389/fnana.2015.00126

Neuropeptide S-and Neuropeptide S receptor-expressing neuron populations in the human pons. / Ádori, C.; Barde, Swapnali; Bogdanovic, Nenad; Uhlén, Mathias; Reinscheid, Rainer R.; Kovacs, Gabor G.; Hökfelt, Tomas.

In: Frontiers in Neuroanatomy, Vol. 9, No. September, A126, 25.09.2015.

Research output: Contribution to journalArticle

Ádori, C, Barde, S, Bogdanovic, N, Uhlén, M, Reinscheid, RR, Kovacs, GG & Hökfelt, T 2015, 'Neuropeptide S-and Neuropeptide S receptor-expressing neuron populations in the human pons', Frontiers in Neuroanatomy, vol. 9, no. September, A126. https://doi.org/10.3389/fnana.2015.00126
Ádori, C. ; Barde, Swapnali ; Bogdanovic, Nenad ; Uhlén, Mathias ; Reinscheid, Rainer R. ; Kovacs, Gabor G. ; Hökfelt, Tomas. / Neuropeptide S-and Neuropeptide S receptor-expressing neuron populations in the human pons. In: Frontiers in Neuroanatomy. 2015 ; Vol. 9, No. September.
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