Neuropeptide localization in the 'migraine generator' region of the human brainstem

J. Tajti, R. Uddman, L. Edvinsson

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Evidence from animals and humans suggests that brainstem nuclei such as the raphe nuclei, the locus coeruleus (LC) and the periaqueductal grey matter (PAG), are involved in the pathophysiology of migraine. In order to understand possible neurotransmitters involved we have, by means of indirect immunocytochemistry, analysed these regions for the occurrence and distribution of calcitonin gene-related peptide (CGRP), substance P (SP), pituitary adenylate-cyclase activating peptide (PACAP) and vasoactive intestinal polypeptide (VIP). CGRP-immunoreactive (-ir) cell bodies, but no fibres, were found to occur in high numbers, constituting 80% of all nerve cell bodies in the LC. A smaller number of these nerve cell bodies (40%) in the LC proved to be PACAP-ir. The LC neurones also stored the vesicular monoamine transporter (VMAT)- and the C-terminal flanking peptide of neuropeptide Y (C-PON)-ir, illustrating their adrenergic nature. Double immunostaining revealed that all VMAT-and C-PON-containing neurones, in addition, stored CGRP. Immunoreactive cell bodies were not seen in the nucleus raphe magnus (NRM) or PAG. Numerous SP-ir nerve fibres were observed in the NRM, the LC and the PAG. Few PACAP-ir nerve fibres were detected in the PAG and few VIP-ir nerve fibres were seen in the NRM and the PAG.

Original languageEnglish
Pages (from-to)96-101
Number of pages6
JournalCephalalgia
Volume21
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Periaqueductal Gray
Locus Coeruleus
Neuropeptides
Migraine Disorders
Brain Stem
Calcitonin Gene-Related Peptide
Vesicular Monoamine Transport Proteins
Nerve Fibers
Adenylyl Cyclases
Neurons
Vasoactive Intestinal Peptide
Substance P
Peptides
Raphe Nuclei
Adrenergic Agents
Neurotransmitter Agents
Immunohistochemistry
Cell Body
Nucleus Raphe Magnus
neuropeptide Y C-terminal flanking peptide

Keywords

  • Immunocytochemistry
  • Locus coeruleus
  • Migraine
  • Neuropeptides
  • Periaqueductal grey
  • Raphe nuclei

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Neuropeptide localization in the 'migraine generator' region of the human brainstem. / Tajti, J.; Uddman, R.; Edvinsson, L.

In: Cephalalgia, Vol. 21, No. 2, 2001, p. 96-101.

Research output: Contribution to journalArticle

Tajti, J. ; Uddman, R. ; Edvinsson, L. / Neuropeptide localization in the 'migraine generator' region of the human brainstem. In: Cephalalgia. 2001 ; Vol. 21, No. 2. pp. 96-101.
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