Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer’s disease and neocortical Lewy body disease

János Bencze, Máté Szarka, Viktor Bencs, Renáta Nóra Szabó, Máté Smajda, Dag Aarsland, Tibor Hortobágyi

Research output: Contribution to journalArticle

Abstract

Alzheimer’s disease (AD) and neocortical Lewy body disease (LBD) are the most common neurodegenerative dementias, with no available curative treatment. Elucidating pathomechanism and identifying novel therapeutic targets are of paramount importance. Lemur tyrosine kinase 2 (LMTK2) is involved in several physiological and pathological cellular processes. Herewith a neuropathological characterization is presented in AD and neocortical LBD samples using chromogenic and fluorescent LMTK2 immunohistochemistry on post-mortem brain tissues and compared them to age-matched controls (CNTs). LMTK2 immunopositivity was limited to the neuronal cytoplasm. Neurons, including tau-positive tangle-bearing ones, showed decreased chromogenic and immunofluorescent labelling in AD in every cortical layer compared to CNT and neocortical LBD. Digital image analysis was performed to measure the average immunopositivity of groups. Mean grey values were calculated for each group after measuring the grey scale LMTK2 signal intensity of each individual neuron. There was significant difference between the mean grey values of CNT vs. AD and neocortical LBD vs. AD. The moderate decrease in neocortical LBD suggests the effect of coexisting AD pathology. We provide neuropathological evidence on decreased neuronal LMTK2 immunolabelling in AD, with implications for pathogenesis.

Original languageEnglish
Article number17222
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Lemur
TYK2 Kinase
Lewy Body Disease
Alzheimer Disease
Neurons
Pathologic Processes
Dementia
Cytoplasm
Immunohistochemistry
Pathology
Brain

ASJC Scopus subject areas

  • General

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Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer’s disease and neocortical Lewy body disease. / Bencze, János; Szarka, Máté; Bencs, Viktor; Szabó, Renáta Nóra; Smajda, Máté; Aarsland, Dag; Hortobágyi, Tibor.

In: Scientific reports, Vol. 9, No. 1, 17222, 01.12.2019.

Research output: Contribution to journalArticle

Bencze, János ; Szarka, Máté ; Bencs, Viktor ; Szabó, Renáta Nóra ; Smajda, Máté ; Aarsland, Dag ; Hortobágyi, Tibor. / Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer’s disease and neocortical Lewy body disease. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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