Neuronal nitric oxide synthase inhibitor has a neuroprotective effect in a rat model of brain injury

Christoph Görlach, Tibor Hortobágyi, Szabolcs Hortobágyi, Zoltán Benyó

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: The aim of the present study was to assess the effects of neuronal nitric oxide synthase (NOS I) inhibitors and a combination of NOS I and NOS II inhibitors on lesion volume after experimental brain injury. Methods: Cold lesion of the brain was induced by application of a precooled (-78 °C) copper cylinder to the intact dura of the rat for 6 s. Brains were removed 24 h after the injury and lesion volume determined using the triphenyltetrazolium-chloride method. Results: The specific NOS I inhibitor 3-bromo-7-nitroindazole (Br-7-NI) reduced lesion volume significantly by 21% compared with the vehicle control. In contrast, 7-nitroindazole had no effect on lesion volume. When aminoguanidine, a specific NOS II inhibitor, was administered after Br-7-NI, lesion volume was significantly reduced but not significantly more than with either compound alone. Conclusion: Brain injury after cold lesion is partly mediated by NOS I activity and can be attenuated successfully with Br-7-NI, while coinhibition of NOS II does not improve the outcome significantly.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalRestorative neurology and neuroscience
Volume17
Issue number2-3
Publication statusPublished - Dec 1 2000

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Keywords

  • 3-Bromo-7-nitroindazole
  • 7-Nitroindazole
  • Aminoguanidine
  • Cold brain injury
  • NOS I
  • NOS II
  • Nitric oxide synthase
  • Rat

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Clinical Neurology

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