Neuronal nitric oxide synthase in the cerebrovascular endothelium

Zoltán Benyó, Z. Lacza, Tibor Hortobágyi, Christoph Görlach, Péter Sándor, Michael Wahl

Research output: Contribution to journalArticle

Abstract

The presence of the neuronal isoform of nitric oxide synthase (nNOS) in astrocytes and neurons as well as in the perivascular nerves and cerebrovascular endothelium is well documented. The role of the nNOS, however, is not yet understood. In the present study, the function of cerebrovascular nNOS was investigated by comparing the effects of the specific nNOS blocker 7-nitro indazole monosodium salt (7-NINA) to that of the general NOS inhibitor NG-nitro-L-arginine (L-NA) in isolated rat basilar arteries (BAs). 7-NINA had no significant effect on the resting tone of the vessels, while L-NA induced strong contraction. The relaxant effect of bradykinin was attenuated in the presence of L-NA but was not changed by 7-NINA. In contrast, 7-NINA markedly reduced the acetylcholine-induced, endothelium-dependent relaxation. These results demonstrate that nNOS contributes significantly to the relaxant effect of acetylcholine, indicating the functional importance of this isoenzyme in the cerebrovascular endothelium.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalInternational Congress Series
Volume1235
Issue numberC
DOIs
Publication statusPublished - Jul 1 2002

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Nitric Oxide Synthase Type I
Endothelium
Protein Isoforms
Salts
Acetylcholine
Basilar Artery
Nitroarginine
Bradykinin
Astrocytes
Isoenzymes
Neurons
7-nitroindazole

Keywords

  • Acetylcholine
  • Bradykinin
  • Endothelium
  • Neuronal nitric oxide synthase
  • Rat basilar artery

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Neuronal nitric oxide synthase in the cerebrovascular endothelium. / Benyó, Zoltán; Lacza, Z.; Hortobágyi, Tibor; Görlach, Christoph; Sándor, Péter; Wahl, Michael.

In: International Congress Series, Vol. 1235, No. C, 01.07.2002, p. 369-377.

Research output: Contribution to journalArticle

Benyó, Z, Lacza, Z, Hortobágyi, T, Görlach, C, Sándor, P & Wahl, M 2002, 'Neuronal nitric oxide synthase in the cerebrovascular endothelium', International Congress Series, vol. 1235, no. C, pp. 369-377. https://doi.org/10.1016/S0531-5131(02)00208-X
Benyó, Zoltán ; Lacza, Z. ; Hortobágyi, Tibor ; Görlach, Christoph ; Sándor, Péter ; Wahl, Michael. / Neuronal nitric oxide synthase in the cerebrovascular endothelium. In: International Congress Series. 2002 ; Vol. 1235, No. C. pp. 369-377.
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