Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism

Sungro Jo, I. Kalló, Zsuzsanna Bardóczi, Rafael Arrojo e Drigo, A. Zeöld, Z. Liposits, Anthony Oliva, Vance P. Lemmon, John L. Bixby, B. Gereben, Antonio C. Bianco

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In neurons, the type 3 deiodinase (D3) inactivates thyroid hormone and reduces oxygen consumption, thus creating a state of cell-specific hypothyroidism. Here we show that hypoxia leads to nuclear import of D3 in neurons, without which thyroid hormone signaling and metabolism cannot be reduced. After unilateral hypoxia in the rat brain, D3 protein level is increased predominantly in the nucleus of the neurons in the pyramidal and granular ipsilateral layers, as well as in the hilus of the dentate gyrus of the hippocampal formation. In hippocampal neurons in culture as well as in a human neuroblastoma cell line (SK-N-AS), a 24 h hypoxia period redirects active D3 from the endoplasmic reticulum to the nucleus via the cochaperone Hsp40 pathway. Preventing nuclear D3 import by Hsp40 knockdown resulted an almost doubling in the thyroid hormone-dependent glycolytic rate and quadrupling the transcription of thyroid hormone target gene ENPP2. In contrast, Hsp40 overexpression increased nuclear import of D3 and minimized thyroid hormone effects in cell metabolism. In conclusion, ischemia/hypoxia induces an Hsp40-mediated translocation of D3 to the nucleus, facilitating thyroid hormone inactivation proximal to the thyroid hormone receptors. This adaptation decreases thyroid hormone signaling and may function to reduce ischemia-induced hypoxic brain damage.

Original languageEnglish
Pages (from-to)8491-8500
Number of pages10
JournalJournal of Neuroscience
Volume32
Issue number25
DOIs
Publication statusPublished - Jun 20 2012

Fingerprint

Iodide Peroxidase
Cell Nucleus Active Transport
Thyroid Hormones
Neurons
Ischemia
Brain Hypoxia
Thyroid Hormone Receptors
Pyramidal Cells
Dentate Gyrus
Hypothyroidism
Hypoxia
Neuroblastoma
Oxygen Consumption
Endoplasmic Reticulum
Hippocampus
Cell Line
Brain
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism. / Jo, Sungro; Kalló, I.; Bardóczi, Zsuzsanna; e Drigo, Rafael Arrojo; Zeöld, A.; Liposits, Z.; Oliva, Anthony; Lemmon, Vance P.; Bixby, John L.; Gereben, B.; Bianco, Antonio C.

In: Journal of Neuroscience, Vol. 32, No. 25, 20.06.2012, p. 8491-8500.

Research output: Contribution to journalArticle

Jo, Sungro ; Kalló, I. ; Bardóczi, Zsuzsanna ; e Drigo, Rafael Arrojo ; Zeöld, A. ; Liposits, Z. ; Oliva, Anthony ; Lemmon, Vance P. ; Bixby, John L. ; Gereben, B. ; Bianco, Antonio C. / Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 25. pp. 8491-8500.
@article{085eceb6604e41d7b831df8366986495,
title = "Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism",
abstract = "In neurons, the type 3 deiodinase (D3) inactivates thyroid hormone and reduces oxygen consumption, thus creating a state of cell-specific hypothyroidism. Here we show that hypoxia leads to nuclear import of D3 in neurons, without which thyroid hormone signaling and metabolism cannot be reduced. After unilateral hypoxia in the rat brain, D3 protein level is increased predominantly in the nucleus of the neurons in the pyramidal and granular ipsilateral layers, as well as in the hilus of the dentate gyrus of the hippocampal formation. In hippocampal neurons in culture as well as in a human neuroblastoma cell line (SK-N-AS), a 24 h hypoxia period redirects active D3 from the endoplasmic reticulum to the nucleus via the cochaperone Hsp40 pathway. Preventing nuclear D3 import by Hsp40 knockdown resulted an almost doubling in the thyroid hormone-dependent glycolytic rate and quadrupling the transcription of thyroid hormone target gene ENPP2. In contrast, Hsp40 overexpression increased nuclear import of D3 and minimized thyroid hormone effects in cell metabolism. In conclusion, ischemia/hypoxia induces an Hsp40-mediated translocation of D3 to the nucleus, facilitating thyroid hormone inactivation proximal to the thyroid hormone receptors. This adaptation decreases thyroid hormone signaling and may function to reduce ischemia-induced hypoxic brain damage.",
author = "Sungro Jo and I. Kall{\'o} and Zsuzsanna Bard{\'o}czi and {e Drigo}, {Rafael Arrojo} and A. Ze{\"o}ld and Z. Liposits and Anthony Oliva and Lemmon, {Vance P.} and Bixby, {John L.} and B. Gereben and Bianco, {Antonio C.}",
year = "2012",
month = "6",
day = "20",
doi = "10.1523/JNEUROSCI.6514-11.2012",
language = "English",
volume = "32",
pages = "8491--8500",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "25",

}

TY - JOUR

T1 - Neuronal hypoxia induces Hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism

AU - Jo, Sungro

AU - Kalló, I.

AU - Bardóczi, Zsuzsanna

AU - e Drigo, Rafael Arrojo

AU - Zeöld, A.

AU - Liposits, Z.

AU - Oliva, Anthony

AU - Lemmon, Vance P.

AU - Bixby, John L.

AU - Gereben, B.

AU - Bianco, Antonio C.

PY - 2012/6/20

Y1 - 2012/6/20

N2 - In neurons, the type 3 deiodinase (D3) inactivates thyroid hormone and reduces oxygen consumption, thus creating a state of cell-specific hypothyroidism. Here we show that hypoxia leads to nuclear import of D3 in neurons, without which thyroid hormone signaling and metabolism cannot be reduced. After unilateral hypoxia in the rat brain, D3 protein level is increased predominantly in the nucleus of the neurons in the pyramidal and granular ipsilateral layers, as well as in the hilus of the dentate gyrus of the hippocampal formation. In hippocampal neurons in culture as well as in a human neuroblastoma cell line (SK-N-AS), a 24 h hypoxia period redirects active D3 from the endoplasmic reticulum to the nucleus via the cochaperone Hsp40 pathway. Preventing nuclear D3 import by Hsp40 knockdown resulted an almost doubling in the thyroid hormone-dependent glycolytic rate and quadrupling the transcription of thyroid hormone target gene ENPP2. In contrast, Hsp40 overexpression increased nuclear import of D3 and minimized thyroid hormone effects in cell metabolism. In conclusion, ischemia/hypoxia induces an Hsp40-mediated translocation of D3 to the nucleus, facilitating thyroid hormone inactivation proximal to the thyroid hormone receptors. This adaptation decreases thyroid hormone signaling and may function to reduce ischemia-induced hypoxic brain damage.

AB - In neurons, the type 3 deiodinase (D3) inactivates thyroid hormone and reduces oxygen consumption, thus creating a state of cell-specific hypothyroidism. Here we show that hypoxia leads to nuclear import of D3 in neurons, without which thyroid hormone signaling and metabolism cannot be reduced. After unilateral hypoxia in the rat brain, D3 protein level is increased predominantly in the nucleus of the neurons in the pyramidal and granular ipsilateral layers, as well as in the hilus of the dentate gyrus of the hippocampal formation. In hippocampal neurons in culture as well as in a human neuroblastoma cell line (SK-N-AS), a 24 h hypoxia period redirects active D3 from the endoplasmic reticulum to the nucleus via the cochaperone Hsp40 pathway. Preventing nuclear D3 import by Hsp40 knockdown resulted an almost doubling in the thyroid hormone-dependent glycolytic rate and quadrupling the transcription of thyroid hormone target gene ENPP2. In contrast, Hsp40 overexpression increased nuclear import of D3 and minimized thyroid hormone effects in cell metabolism. In conclusion, ischemia/hypoxia induces an Hsp40-mediated translocation of D3 to the nucleus, facilitating thyroid hormone inactivation proximal to the thyroid hormone receptors. This adaptation decreases thyroid hormone signaling and may function to reduce ischemia-induced hypoxic brain damage.

UR - http://www.scopus.com/inward/record.url?scp=84862888248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862888248&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.6514-11.2012

DO - 10.1523/JNEUROSCI.6514-11.2012

M3 - Article

C2 - 22723689

AN - SCOPUS:84862888248

VL - 32

SP - 8491

EP - 8500

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 25

ER -