Neuronal background of activation of estivated snails, with special attention to the monoaminergic system: A biochemical, physiological, and neuroanatomical study

L. Hernádi, A. Vehovszky, J. Gyori, L. Hiripi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Osmotic stimulation activates both estivated and inactivated specimens of Helix pomatia and increases their central arousal. High-pressure liquid chromatography has shown that, during activation, the level of both serotonin and dopamine decreases in the central nervous system (CNS) but increases in the foot and heart, organs that are involved in the eversion of the body. In isolated CNS from activated animals, the firing frequency of the heart-modulator serotonergic (RPas) neurons is significantly higher than that in the CNS of estivated or inactivated animals. These neurons innervate both the heart and the anterior aorta. In semi-intact preparations, distilled water (an osmotic stimulus) applied to the mantle collar increases their firing frequency, whereas tactile stimulation evokes their inhibition. Extracellularly applied monoamines mimic the effect of peripheral stimuli: serotonin (0.1-10 μM) increases the activity of the RPas neurons, whereas dopamine (0.1-10 μM) inhibits their activity. Tyrosine-hydroxylase immunocytochemistry and retrograde neurobiotin tracing have revealed similar bipolar receptor cells in the mantle collar and tail, organs that are exposed to environmental stimuli in estivated animals. Serotonin immunocytochemistry carried out on the same tissues does not visualize receptor cells but labels a dense network of fibers that appear to innervate neurobiotin-labeled receptor cells. The combination of neurobiotin-labeling of RPas neurons and immunolabeling suggests that RPas neurons receive direct dopaminergic inputs from receptor cells and serotonergic inputs from central serotonergic neurons, indicating that central serotonergic neurons are interconnected. Thus, the RPas neurons may belong to neuronal elements of the arousal system.

Original languageEnglish
Pages (from-to)539-553
Number of pages15
JournalCell and Tissue Research
Volume331
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Snails
Serotonergic Neurons
Neurons
Chemical activation
Serotonin
Central Nervous System
Arousal
Neurology
Immunohistochemistry
Animals
Dopaminergic Neurons
Touch
Tyrosine 3-Monooxygenase
Aorta
Tail
Foot
Dopamine
High Pressure Liquid Chromatography
High pressure liquid chromatography
Water

Keywords

  • Arousal
  • Dopamine
  • Helix pomatia (Mollusca)
  • Immunocytochemistry
  • Serotonin

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

@article{17a563b0ede747ab94877181318c9e5f,
title = "Neuronal background of activation of estivated snails, with special attention to the monoaminergic system: A biochemical, physiological, and neuroanatomical study",
abstract = "Osmotic stimulation activates both estivated and inactivated specimens of Helix pomatia and increases their central arousal. High-pressure liquid chromatography has shown that, during activation, the level of both serotonin and dopamine decreases in the central nervous system (CNS) but increases in the foot and heart, organs that are involved in the eversion of the body. In isolated CNS from activated animals, the firing frequency of the heart-modulator serotonergic (RPas) neurons is significantly higher than that in the CNS of estivated or inactivated animals. These neurons innervate both the heart and the anterior aorta. In semi-intact preparations, distilled water (an osmotic stimulus) applied to the mantle collar increases their firing frequency, whereas tactile stimulation evokes their inhibition. Extracellularly applied monoamines mimic the effect of peripheral stimuli: serotonin (0.1-10 μM) increases the activity of the RPas neurons, whereas dopamine (0.1-10 μM) inhibits their activity. Tyrosine-hydroxylase immunocytochemistry and retrograde neurobiotin tracing have revealed similar bipolar receptor cells in the mantle collar and tail, organs that are exposed to environmental stimuli in estivated animals. Serotonin immunocytochemistry carried out on the same tissues does not visualize receptor cells but labels a dense network of fibers that appear to innervate neurobiotin-labeled receptor cells. The combination of neurobiotin-labeling of RPas neurons and immunolabeling suggests that RPas neurons receive direct dopaminergic inputs from receptor cells and serotonergic inputs from central serotonergic neurons, indicating that central serotonergic neurons are interconnected. Thus, the RPas neurons may belong to neuronal elements of the arousal system.",
keywords = "Arousal, Dopamine, Helix pomatia (Mollusca), Immunocytochemistry, Serotonin",
author = "L. Hern{\'a}di and A. Vehovszky and J. Gyori and L. Hiripi",
year = "2008",
month = "2",
doi = "10.1007/s00441-007-0522-3",
language = "English",
volume = "331",
pages = "539--553",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Neuronal background of activation of estivated snails, with special attention to the monoaminergic system

T2 - A biochemical, physiological, and neuroanatomical study

AU - Hernádi, L.

AU - Vehovszky, A.

AU - Gyori, J.

AU - Hiripi, L.

PY - 2008/2

Y1 - 2008/2

N2 - Osmotic stimulation activates both estivated and inactivated specimens of Helix pomatia and increases their central arousal. High-pressure liquid chromatography has shown that, during activation, the level of both serotonin and dopamine decreases in the central nervous system (CNS) but increases in the foot and heart, organs that are involved in the eversion of the body. In isolated CNS from activated animals, the firing frequency of the heart-modulator serotonergic (RPas) neurons is significantly higher than that in the CNS of estivated or inactivated animals. These neurons innervate both the heart and the anterior aorta. In semi-intact preparations, distilled water (an osmotic stimulus) applied to the mantle collar increases their firing frequency, whereas tactile stimulation evokes their inhibition. Extracellularly applied monoamines mimic the effect of peripheral stimuli: serotonin (0.1-10 μM) increases the activity of the RPas neurons, whereas dopamine (0.1-10 μM) inhibits their activity. Tyrosine-hydroxylase immunocytochemistry and retrograde neurobiotin tracing have revealed similar bipolar receptor cells in the mantle collar and tail, organs that are exposed to environmental stimuli in estivated animals. Serotonin immunocytochemistry carried out on the same tissues does not visualize receptor cells but labels a dense network of fibers that appear to innervate neurobiotin-labeled receptor cells. The combination of neurobiotin-labeling of RPas neurons and immunolabeling suggests that RPas neurons receive direct dopaminergic inputs from receptor cells and serotonergic inputs from central serotonergic neurons, indicating that central serotonergic neurons are interconnected. Thus, the RPas neurons may belong to neuronal elements of the arousal system.

AB - Osmotic stimulation activates both estivated and inactivated specimens of Helix pomatia and increases their central arousal. High-pressure liquid chromatography has shown that, during activation, the level of both serotonin and dopamine decreases in the central nervous system (CNS) but increases in the foot and heart, organs that are involved in the eversion of the body. In isolated CNS from activated animals, the firing frequency of the heart-modulator serotonergic (RPas) neurons is significantly higher than that in the CNS of estivated or inactivated animals. These neurons innervate both the heart and the anterior aorta. In semi-intact preparations, distilled water (an osmotic stimulus) applied to the mantle collar increases their firing frequency, whereas tactile stimulation evokes their inhibition. Extracellularly applied monoamines mimic the effect of peripheral stimuli: serotonin (0.1-10 μM) increases the activity of the RPas neurons, whereas dopamine (0.1-10 μM) inhibits their activity. Tyrosine-hydroxylase immunocytochemistry and retrograde neurobiotin tracing have revealed similar bipolar receptor cells in the mantle collar and tail, organs that are exposed to environmental stimuli in estivated animals. Serotonin immunocytochemistry carried out on the same tissues does not visualize receptor cells but labels a dense network of fibers that appear to innervate neurobiotin-labeled receptor cells. The combination of neurobiotin-labeling of RPas neurons and immunolabeling suggests that RPas neurons receive direct dopaminergic inputs from receptor cells and serotonergic inputs from central serotonergic neurons, indicating that central serotonergic neurons are interconnected. Thus, the RPas neurons may belong to neuronal elements of the arousal system.

KW - Arousal

KW - Dopamine

KW - Helix pomatia (Mollusca)

KW - Immunocytochemistry

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=38349044245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349044245&partnerID=8YFLogxK

U2 - 10.1007/s00441-007-0522-3

DO - 10.1007/s00441-007-0522-3

M3 - Article

C2 - 17973120

AN - SCOPUS:38349044245

VL - 331

SP - 539

EP - 553

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 2

ER -