Neural stem cell spreading on lipid based artificial cell surfaces, characterized by combined X-ray and neutron reflectometry

Martin Huth, Samira Hertrich, G. Mező, E. Madarász, Bert Nickel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We developed a bioadhesive coating based on a synthetic peptide-conjugate (AK-cyclo[RGDfC]) which contains multiples of the arginyl-glycyl-aspartic acid (RGD) amino acid sequence. Biotinylated AK-cyclo[RGDfC] is bound to a supported lipid bilayer via a streptavidin interlayer. Layering, hydration and packing of the coating is quantified by X-ray and neutron reflectometry experiments. AK-cyclo[RGDfC] binds to the streptavidin interlayer in a stretched-out on edge configuration. The highly packed configuration with only 12% water content maximizes the number of accessible adhesion sites. Enhanced cell spreading of neural stem cells was observed for AK-cyclo[RGDfC] functionalized bilayers. Due to the large variety of surfaces which can be coated by physisorption of lipid bilayers, this approach is of general interest for the fabrication of biocompatible surfaces.

Original languageEnglish
Pages (from-to)4994-5006
Number of pages13
JournalMaterials
Volume3
Issue number11
DOIs
Publication statusPublished - 2010

Fingerprint

Lipid bilayers
Stem cells
Lipids
Neutrons
X rays
Coatings
Physisorption
Streptavidin
Hydration
Water content
Peptides
Amino acids
Adhesion
Fabrication
Acids
Experiments
Amino Acids
cyclo(arginyl-glycyl-aspartyl-phenylalanyl-cysteinyl)

Keywords

  • Biomimetics
  • Bionanotechnology
  • Functional coatings
  • Self-assembly
  • Supported lipid bilayers

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Neural stem cell spreading on lipid based artificial cell surfaces, characterized by combined X-ray and neutron reflectometry. / Huth, Martin; Hertrich, Samira; Mező, G.; Madarász, E.; Nickel, Bert.

In: Materials, Vol. 3, No. 11, 2010, p. 4994-5006.

Research output: Contribution to journalArticle

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