Nesterenkonia pannonica sp. Nov., a novel alkaliphilic and moderately halophilic actinobacterium

A. Borsodi, Tibor Szili-Kovács, Peter Schumann, Cathrin Spröer, K. Márialigeti, E. Tóth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An alkaliphilic and moderately halophilic bacterial strain characterized by optimal growth at pH 9.0–10.0 and with 5–7% (w/v) NaCl, designated BV-35T, was isolated from water of a soda pan located in Kiskunság National Park, Hungary. Cells of the orange-pigmented colony were Gram-stain-positive, non-motile and non-endospore-forming coccoid rods. The isolate was strictly aerobic, catalase-positive and oxidase-negative. Strain BV-35T displayed a peptidoglycan similar to type A4a, L-Lys–L-Glu (A11.54 according to www.peptidoglycan-types.info) but containing additionally 4-aminobutyric acid. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone, and anteiso-C15: 0 and anteiso-C17: 0 were its major cellular fatty acids. The DNA G+C content of strain BV-35T was 65.4 mol%. Based on 16S rRNA gene sequence similarities, the novel isolate showed the closest relationship to Nesterenkonia populi GP 10-3T (97.9 %). The DNA–DNA relatedness between BV-35T and N. populi was 46.7 %. The distinguishing phenotypic and genetic results of this polyphasic study revealed that strain BV-35T represents a novel member of the genus Nesterenkonia, for which the name Nesterenkonia pannonica sp. Nov. is proposed. The type strain is BV-35T (=DSM 29786T=NCAIM B 02606T).

Original languageEnglish
Article number002263
Pages (from-to)4116-4120
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Nesterenkonia
Peptidoglycan
Actinobacteria
isoprenoid
relatedness
Carbonated Water
national park
fatty acid
DNA
Hungary
gene
acid
Terpenes
Base Composition
rRNA Genes
Catalase
gamma-Aminobutyric Acid
peptidoglycans
Names
Oxidoreductases

Keywords

  • Alkaliphilic
  • Moderately halophilic
  • Nesterenkonia
  • Soda pan

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Nesterenkonia pannonica sp. Nov., a novel alkaliphilic and moderately halophilic actinobacterium. / Borsodi, A.; Szili-Kovács, Tibor; Schumann, Peter; Spröer, Cathrin; Márialigeti, K.; Tóth, E.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 10, 002263, 01.10.2017, p. 4116-4120.

Research output: Contribution to journalArticle

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AU - Borsodi, A.

AU - Szili-Kovács, Tibor

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AU - Márialigeti, K.

AU - Tóth, E.

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