Neoplastic transformation and lineage switching of rat liver epithelial cells by retrovirus-associated oncogenes

S. Garfield, B. E. Huber, P. Nagy, M. G. Cordingley, S. S. Thorgeirsson

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Abstract

Tumors produced by a chemically transformed rat liver epithelial (RLE) cell line and its single cell-derived clonal subpopulations demonstrate wide-ranging morphological presentations including carcinomas, sarcomas, 'mixed epithelial-mesenchymal' tumors, and undifferentiated tumors [Am J Pathol 127:168-181, 1987]. To address the question of heterogeneity of tumors derived from transformed RLE cells, we have used recombinant retroviruses containing the following transforming oncogenes: v-raf (3611-MSV), v-raf/v-myc (J2), v-myc (J5), and v-Ha-ras (pRNR16). All of the oncogenes, with the exception of v-myc (J5), were efficient transforming agents in the RLE cells. Tumors derived from the v-raf- and, to a lesser extent, those from v-Ha-ras-transformed RLE cells showed mixed epithelial-mesenchymal morphology whereas the combination of v-raf/v-myc (J2) consistently produced differentiated trabecular carcinomas. These data suggest that the lineage commitment of the RLE cells can be perturbed by a single transforming oncogene and that different tumor types derived from these cells may reflect the expression of a selective oncogene or a combination of oncogenes.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalMolecular Carcinogenesis
Volume1
Issue number3
Publication statusPublished - 1988

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Retroviridae
Oncogenes
Epithelial Cells
Liver
Neoplasms
Carcinoma
Sarcoma
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

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Neoplastic transformation and lineage switching of rat liver epithelial cells by retrovirus-associated oncogenes. / Garfield, S.; Huber, B. E.; Nagy, P.; Cordingley, M. G.; Thorgeirsson, S. S.

In: Molecular Carcinogenesis, Vol. 1, No. 3, 1988, p. 189-195.

Research output: Contribution to journalArticle

Garfield, S, Huber, BE, Nagy, P, Cordingley, MG & Thorgeirsson, SS 1988, 'Neoplastic transformation and lineage switching of rat liver epithelial cells by retrovirus-associated oncogenes', Molecular Carcinogenesis, vol. 1, no. 3, pp. 189-195.
Garfield, S. ; Huber, B. E. ; Nagy, P. ; Cordingley, M. G. ; Thorgeirsson, S. S. / Neoplastic transformation and lineage switching of rat liver epithelial cells by retrovirus-associated oncogenes. In: Molecular Carcinogenesis. 1988 ; Vol. 1, No. 3. pp. 189-195.
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