Neoplastic transformation and lineage switching of rat liver epithelial cells by retrovirus‐associated oncogenes

Susan Garfield, Brian E. Huber, Peter Nagy, Michael G. Cordingley, Snorri S. Thorgeirsson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Tumors produced by a chemically transformed rat liver epithelial (RLE) cell line and its single cell‐derived clonal subpopulations demonstrate wide‐ranging morphological presentations including carcinomas, sarcomas, “mixed epithelial‐mesenchymal” tumors, and undifferentiated tumors [Am J Pathol 127:168–181, 1987]. To address the question of heterogeneity of tumors derived from transformed RLE cells, we have used recombinant retroviruses containing the following transforming oncogenes: v‐raf (3611‐MSV), v‐raf/v‐myc (J2), v‐myc (J5), and v‐Ha‐ras (pRNR16). All of the oncogenes, with the exception of v‐myc (J5), were efficient transforming agents in the RLE cells. Tumors derived from the v‐raf‐ and, to a lesser extent, those from v‐Ha‐ras—transformed RLE cells showed mixed epithelial‐mesenchymal morphology, whereas the combination of v‐raf/v‐myc (J2) consistently produced differentiated trabecular carcinomas. These data suggest that the lineage commitment of the RLE cells can be perturbed by a single transforming oncogene and that different tumor types derived from these cells may reflect the expression of a selective oncogene or a combination of oncogenes.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalMolecular Carcinogenesis
Volume1
Issue number3
DOIs
Publication statusPublished - 1988

Keywords

  • Key words
  • Oncogenes
  • epithelial cells
  • lineage switching
  • liver

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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