Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

A. Vehovszky, A. Farkas, A. Ács, O. Stoliar, A. Székács, M. Mörtl, J. Gyori

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Neonicotinoids are highly potent and selective systemic insecticides, but their widespread use also has a growing impact on non-target animals and contaminates the environment, including surface waters. We tested the neonicotinoid insecticides commercially available in Hungary (acetamiprid, Mospilan; imidacloprid, Kohinor thiamethoxam, Actara; clothianidin, Apacs; thiacloprid, Calypso) on cholinergic synapses that exist between the VD4 and RPeD1 neurons in the central nervous system of the pond snail Lymnaea stagnalis. In the concentration range used (0.01-1. mg/ml), neither chemical acted as an acetylcholine (ACh) agonist; instead, both displayed antagonist activity, inhibiting the cholinergic excitatory components of the VD4-RPeD1 connection. Thiacloprid (0.01. mg/ml) blocked almost 90% of excitatory postsynaptic potentials (EPSPs), while the less effective thiamethoxam (0.1. mg/ml) reduced the synaptic responses by about 15%. The ACh-evoked membrane responses of the RPeD1 neuron were similarly inhibited by the neonicotinoids, confirming that the same ACh receptor (AChR) target was involved. We conclude that neonicotinoids act on nicotinergic acetylcholine receptors (nAChRs) in the snail CNS. This has been established previously in the insect CNS; however, our data indicate differences in the background mechanism or the nAChR binding site in the snail.Here, we provide the first results concerning neonicotinoid-related toxic effects on the neuronal connections in the molluscan nervous system. Aquatic animals, including molluscs, are at direct risk while facing contaminated surface waters, and snails may provide a suitable model for further studies of the behavioral/neuronal consequences of intoxication by neonicotinoids.

Original languageEnglish
Article number4181
Pages (from-to)172-179
Number of pages8
JournalAquatic Toxicology
Volume167
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Lymnaea
Lymnaea stagnalis
neonicotinoid insecticides
cholinergic agents
Snails
nervous system
Insecticides
Synaptic Transmission
Cholinergic Agents
Nervous System
insecticide
snail
snails
Cholinergic Receptors
thiamethoxam
thiacloprid
cholinergic receptors
acetylcholine
Neurons
Cholinergic Agonists

Keywords

  • Acetamiprid
  • Clothianidin
  • Imidacloprid
  • Neuron
  • Thiacloprid
  • Thiamethoxam

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system. / Vehovszky, A.; Farkas, A.; Ács, A.; Stoliar, O.; Székács, A.; Mörtl, M.; Gyori, J.

In: Aquatic Toxicology, Vol. 167, 4181, 01.10.2015, p. 172-179.

Research output: Contribution to journalArticle

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AU - Székács, A.

AU - Mörtl, M.

AU - Gyori, J.

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