Neoglycoproteins as carbohydrate antigens

Synthesis, analysis, and polyclonal antibody response

Márta Kerékgyártó, Anikó Fekete, Zoltán Szurmai, János Kerékgyártó, László Takács, István Kurucz, A. Guttman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The analysis and polyclonal antibody response for newly synthesized maltose-BSA conjugate neoglycoproteins is described. In this first proof of concept study, a simple carbohydrate antigen, maltose, was linked to BSA by reductive amination. An aglycone spacer was utilized to conserve the intact annular maltose structure and to promote the accessibility of the carbohydrate immunogen hapten during immunization. The neoglycoproteins were investigated by CGE and the number of conjugated maltose residues was determined by MALDI-TOF MS. The neoglycoproteins were then evaluated by immunization of BALB/c mice and the polyclonal antibody response was tested by ELISA as evidence for the presence of sugar-containing epitope-specific antibodies. Selective antibody binding was demonstrated to the synthesized neoglycoproteins with different (low and high) glycosylation degrees suggesting the possible use of this approach to generate antibodies. Moreover, the polyclonal antibody response was not inhibited by maltose or other simple carbohydrates to confirm presence of the neoglycoprotein-specific antibodies.

Original languageEnglish
Pages (from-to)2379-2386
Number of pages8
JournalElectrophoresis
Volume34
Issue number16
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Maltose
Antibody Formation
Glycoproteins
Carbohydrates
Antigens
Antibodies
Immunization
Amination
Haptens
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Glycosylation
Epitopes
Enzyme-Linked Immunosorbent Assay
Sugars

Keywords

  • Carbohydrate antigen
  • Carbohydrate-specific antibody
  • ELISA
  • Neoglyco-protein

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Kerékgyártó, M., Fekete, A., Szurmai, Z., Kerékgyártó, J., Takács, L., Kurucz, I., & Guttman, A. (2013). Neoglycoproteins as carbohydrate antigens: Synthesis, analysis, and polyclonal antibody response. Electrophoresis, 34(16), 2379-2386. https://doi.org/10.1002/elps.201300052

Neoglycoproteins as carbohydrate antigens : Synthesis, analysis, and polyclonal antibody response. / Kerékgyártó, Márta; Fekete, Anikó; Szurmai, Zoltán; Kerékgyártó, János; Takács, László; Kurucz, István; Guttman, A.

In: Electrophoresis, Vol. 34, No. 16, 08.2013, p. 2379-2386.

Research output: Contribution to journalArticle

Kerékgyártó, M, Fekete, A, Szurmai, Z, Kerékgyártó, J, Takács, L, Kurucz, I & Guttman, A 2013, 'Neoglycoproteins as carbohydrate antigens: Synthesis, analysis, and polyclonal antibody response', Electrophoresis, vol. 34, no. 16, pp. 2379-2386. https://doi.org/10.1002/elps.201300052
Kerékgyártó M, Fekete A, Szurmai Z, Kerékgyártó J, Takács L, Kurucz I et al. Neoglycoproteins as carbohydrate antigens: Synthesis, analysis, and polyclonal antibody response. Electrophoresis. 2013 Aug;34(16):2379-2386. https://doi.org/10.1002/elps.201300052
Kerékgyártó, Márta ; Fekete, Anikó ; Szurmai, Zoltán ; Kerékgyártó, János ; Takács, László ; Kurucz, István ; Guttman, A. / Neoglycoproteins as carbohydrate antigens : Synthesis, analysis, and polyclonal antibody response. In: Electrophoresis. 2013 ; Vol. 34, No. 16. pp. 2379-2386.
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