Neocortical c-fos mRNA transcription in repeated, brief, acute seizures: is c-fos a coincidence detector?

A. Mihály, Sándor Borbély, I. Világi, L. Détári, Roland Weiczner, Zsolt Zádor, B. Krisztin-Péva, Andrea Bagosi, Zsolt Kopniczky, E. Zádor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4-aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4-AP treatment. Tissue samples of the somatosensory neocortex were collected at 30 min, 1 h, 3 h, 5 h and 8 h following the treatment for PCR and immunohistochemistry. The c-fos mRNA displayed the first significant rise at 1 h, and remained significantly higher through 3 h. The number of c-fos protein immunoreactive cells was significantly elevated already at 30 min, peaked at 1 h, and declined by 5 h. We conclude that in repetitive, brief seizures, the first convulsion does not increase c-fos RNA transcription, whilst the second causes a long-lasting gene expression and a large increase of c-fos protein synthesis. The phenomenon may have implications in the pathogenesis of human and animal epilepsies.

Original languageEnglish
Pages (from-to)481-486
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume15
Issue number3
Publication statusPublished - Mar 2005

Fingerprint

Proto-Oncogene Proteins c-fos
Seizures
Messenger RNA
Tonic-Clonic Epilepsy
4-Aminopyridine
Implanted Electrodes
Neocortex
Electroencephalography
Epilepsy
Immunohistochemistry
RNA
Gene Expression
Polymerase Chain Reaction
Therapeutics

ASJC Scopus subject areas

  • Genetics

Cite this

Neocortical c-fos mRNA transcription in repeated, brief, acute seizures : is c-fos a coincidence detector? / Mihály, A.; Borbély, Sándor; Világi, I.; Détári, L.; Weiczner, Roland; Zádor, Zsolt; Krisztin-Péva, B.; Bagosi, Andrea; Kopniczky, Zsolt; Zádor, E.

In: International Journal of Molecular Medicine, Vol. 15, No. 3, 03.2005, p. 481-486.

Research output: Contribution to journalArticle

@article{603835b08f80433dab8804604e2d2aaf,
title = "Neocortical c-fos mRNA transcription in repeated, brief, acute seizures: is c-fos a coincidence detector?",
abstract = "The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4-aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4-AP treatment. Tissue samples of the somatosensory neocortex were collected at 30 min, 1 h, 3 h, 5 h and 8 h following the treatment for PCR and immunohistochemistry. The c-fos mRNA displayed the first significant rise at 1 h, and remained significantly higher through 3 h. The number of c-fos protein immunoreactive cells was significantly elevated already at 30 min, peaked at 1 h, and declined by 5 h. We conclude that in repetitive, brief seizures, the first convulsion does not increase c-fos RNA transcription, whilst the second causes a long-lasting gene expression and a large increase of c-fos protein synthesis. The phenomenon may have implications in the pathogenesis of human and animal epilepsies.",
author = "A. Mih{\'a}ly and S{\'a}ndor Borb{\'e}ly and I. Vil{\'a}gi and L. D{\'e}t{\'a}ri and Roland Weiczner and Zsolt Z{\'a}dor and B. Krisztin-P{\'e}va and Andrea Bagosi and Zsolt Kopniczky and E. Z{\'a}dor",
year = "2005",
month = "3",
language = "English",
volume = "15",
pages = "481--486",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "3",

}

TY - JOUR

T1 - Neocortical c-fos mRNA transcription in repeated, brief, acute seizures

T2 - is c-fos a coincidence detector?

AU - Mihály, A.

AU - Borbély, Sándor

AU - Világi, I.

AU - Détári, L.

AU - Weiczner, Roland

AU - Zádor, Zsolt

AU - Krisztin-Péva, B.

AU - Bagosi, Andrea

AU - Kopniczky, Zsolt

AU - Zádor, E.

PY - 2005/3

Y1 - 2005/3

N2 - The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4-aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4-AP treatment. Tissue samples of the somatosensory neocortex were collected at 30 min, 1 h, 3 h, 5 h and 8 h following the treatment for PCR and immunohistochemistry. The c-fos mRNA displayed the first significant rise at 1 h, and remained significantly higher through 3 h. The number of c-fos protein immunoreactive cells was significantly elevated already at 30 min, peaked at 1 h, and declined by 5 h. We conclude that in repetitive, brief seizures, the first convulsion does not increase c-fos RNA transcription, whilst the second causes a long-lasting gene expression and a large increase of c-fos protein synthesis. The phenomenon may have implications in the pathogenesis of human and animal epilepsies.

AB - The effect of acute brief seizures on neocortical c-fos expression was investigated in rats injected with 5 mg/kg 4-aminopyridine. Electroencephalography in freely moving animals with implanted neocortical electrodes detected an average of 2.67 tonic-clonic convulsions within 1 h following the 4-AP treatment. Tissue samples of the somatosensory neocortex were collected at 30 min, 1 h, 3 h, 5 h and 8 h following the treatment for PCR and immunohistochemistry. The c-fos mRNA displayed the first significant rise at 1 h, and remained significantly higher through 3 h. The number of c-fos protein immunoreactive cells was significantly elevated already at 30 min, peaked at 1 h, and declined by 5 h. We conclude that in repetitive, brief seizures, the first convulsion does not increase c-fos RNA transcription, whilst the second causes a long-lasting gene expression and a large increase of c-fos protein synthesis. The phenomenon may have implications in the pathogenesis of human and animal epilepsies.

UR - http://www.scopus.com/inward/record.url?scp=19644361926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19644361926&partnerID=8YFLogxK

M3 - Article

C2 - 15702242

AN - SCOPUS:19644361926

VL - 15

SP - 481

EP - 486

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 3

ER -