Negative association of l-arginine methylation products with oocyte numbers

J. Bódis, Á Várnagy, E. Sulyok, G. L. Kovács, J. Martens-Lobenhoffer, S. M. Bode-Böger

Research output: Contribution to journalArticle

5 Citations (Scopus)


The aim of this study was to determine the concentrations of l-arginine and methylarginines in follicular fluid obtained from women participating in our IVF program and to find clinical correlates of these biochemical parameters. Methods Follicular fluid was obtained from 108 women by ultrasonography guided transvaginal puncture following controlled ovarian hyperstimulation. Follicular fluid l-arginine, asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and monomethylarginine (MMA) concentrations were determined with liquid chromatography-tandem mass spectrometry. The integrated index of arginine methylation (arg-MI) was calculated according to the formula: arg-MI = (ADMA + SDMA)/MMA. Results There were significant inverse relationships between IVF embryo number and follicular fluid l-arginine (r = -0.507, P < 0.001), ADMA (r = -0.356, P < 0.024), SDMA (r = -0.347, P < 0.028), MMA (r = -0.449, P < 0.004) and to l-arginine/ADMA ratio (r = -0.328, P < 0.031). By contrast, arg-MI was directly related to IVF embryo number (r = 0.426, P < 0.006). Moreover, the number of IVF oocytes was also inversely related to ADMA (r = -0.202, P < 0.037) and MMA (r = -0.384, P < 0.012) and positively to arg-MI (r = 0.450, P < 0.03). Conclusions The elevated levels of follicular fluid l-arginine and methylarginines appear to have an adverse influence on the reproductive processes as reflected by a reduction in the number of oocytes and embryos conceived. In contrast, the integrated methylation index proved to be positively correlated to the above parameters of fertilization.

Original languageEnglish
Pages (from-to)3095-3100
Number of pages6
JournalHuman Reproduction
Issue number12
Publication statusPublished - Dec 2010


  • follicular fluid
  • in vitro fertilization
  • l-arginine
  • methylarginines
  • oocyte number

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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