Naturally occurring parvoviral infection in Hungarian broiler flocks

Elena Alina Palade, János Kisary, Zsófia Benyeda, Míra Mándoki, Gyula Balka, Csaba Jakab, Borbála Végh, Zoltán Demeter, M. Rusvai

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The major enteric disease (ED) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. Viruses from numerous families have been identified in the intestinal tracts of poultry with ED, such as Astroviridae, Coronaviridae, Reoviridae, Rotaviridae, and Parvoviridae. The objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (ChPV) and turkey parvovirus (TuPV) in Hungarian flocks experiencing clinical signs of ED. ChPV and TuPV infection were demonstrated in 15 chicken flocks and two turkey flocks, in intestinal samples collected between 2008 and 2010. The histopathological investigation revealed enteritis in the duodenum and jejunum, and atrophy of the lymphoid organs. Indirect immunohistochemistry (IHC) suggested the intestinal epithelium of chickens and turkeys as a potential replication site of the virus, similarly to other parvoviruses, while in case of the turkey samples IHC positivity was also observed in the bursa of Fabricius, liver and pancreas. However, no direct connection could be established between the presence of the pathogen in the above-mentioned tissues and the histopathological changes observed in the investigated flocks. The phylogenetic analysis performed on the partial nucleic acid sequence of the NS1 gene revealed an evident clustering tendency of the ChPV and TuPV strains, but also highlighted the potential reciprocal role of these two species in the epidemiology of these viruses. The role of the ChPV and TuPV in the ED is far from understood, but the results of the present study emphasize the fact that in certain, still not fully elucidated conditions, ChPV and TuPV may participate in the emergence of ED in chicken flocks, as suggested by previous experimental infections.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalAvian Pathology
Volume40
Issue number2
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Parvovirus
Galliform aveparvovirus 1
flocks
Chickens
broiler chickens
Protoparvovirus
digestive system diseases
Infection
infection
Parvoviridae
enteritis
chickens
viruses
immunohistochemistry
Astroviridae
Poult Enteritis Mortality Syndrome
Coronaviridae
Reoviridae
Immunohistochemistry
Growth Disorders

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Cite this

Palade, E. A., Kisary, J., Benyeda, Z., Mándoki, M., Balka, G., Jakab, C., ... Rusvai, M. (2011). Naturally occurring parvoviral infection in Hungarian broiler flocks. Avian Pathology, 40(2), 191-197. https://doi.org/10.1080/03079457.2011.553213

Naturally occurring parvoviral infection in Hungarian broiler flocks. / Palade, Elena Alina; Kisary, János; Benyeda, Zsófia; Mándoki, Míra; Balka, Gyula; Jakab, Csaba; Végh, Borbála; Demeter, Zoltán; Rusvai, M.

In: Avian Pathology, Vol. 40, No. 2, 04.2011, p. 191-197.

Research output: Contribution to journalArticle

Palade, EA, Kisary, J, Benyeda, Z, Mándoki, M, Balka, G, Jakab, C, Végh, B, Demeter, Z & Rusvai, M 2011, 'Naturally occurring parvoviral infection in Hungarian broiler flocks', Avian Pathology, vol. 40, no. 2, pp. 191-197. https://doi.org/10.1080/03079457.2011.553213
Palade EA, Kisary J, Benyeda Z, Mándoki M, Balka G, Jakab C et al. Naturally occurring parvoviral infection in Hungarian broiler flocks. Avian Pathology. 2011 Apr;40(2):191-197. https://doi.org/10.1080/03079457.2011.553213
Palade, Elena Alina ; Kisary, János ; Benyeda, Zsófia ; Mándoki, Míra ; Balka, Gyula ; Jakab, Csaba ; Végh, Borbála ; Demeter, Zoltán ; Rusvai, M. / Naturally occurring parvoviral infection in Hungarian broiler flocks. In: Avian Pathology. 2011 ; Vol. 40, No. 2. pp. 191-197.
@article{0f50dba48c6c4766bce5857c6857b67c,
title = "Naturally occurring parvoviral infection in Hungarian broiler flocks",
abstract = "The major enteric disease (ED) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. Viruses from numerous families have been identified in the intestinal tracts of poultry with ED, such as Astroviridae, Coronaviridae, Reoviridae, Rotaviridae, and Parvoviridae. The objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (ChPV) and turkey parvovirus (TuPV) in Hungarian flocks experiencing clinical signs of ED. ChPV and TuPV infection were demonstrated in 15 chicken flocks and two turkey flocks, in intestinal samples collected between 2008 and 2010. The histopathological investigation revealed enteritis in the duodenum and jejunum, and atrophy of the lymphoid organs. Indirect immunohistochemistry (IHC) suggested the intestinal epithelium of chickens and turkeys as a potential replication site of the virus, similarly to other parvoviruses, while in case of the turkey samples IHC positivity was also observed in the bursa of Fabricius, liver and pancreas. However, no direct connection could be established between the presence of the pathogen in the above-mentioned tissues and the histopathological changes observed in the investigated flocks. The phylogenetic analysis performed on the partial nucleic acid sequence of the NS1 gene revealed an evident clustering tendency of the ChPV and TuPV strains, but also highlighted the potential reciprocal role of these two species in the epidemiology of these viruses. The role of the ChPV and TuPV in the ED is far from understood, but the results of the present study emphasize the fact that in certain, still not fully elucidated conditions, ChPV and TuPV may participate in the emergence of ED in chicken flocks, as suggested by previous experimental infections.",
author = "Palade, {Elena Alina} and J{\'a}nos Kisary and Zs{\'o}fia Benyeda and M{\'i}ra M{\'a}ndoki and Gyula Balka and Csaba Jakab and Borb{\'a}la V{\'e}gh and Zolt{\'a}n Demeter and M. Rusvai",
year = "2011",
month = "4",
doi = "10.1080/03079457.2011.553213",
language = "English",
volume = "40",
pages = "191--197",
journal = "Avian Pathology",
issn = "0307-9457",
publisher = "Taylor and Francis Ltd.",
number = "2",

}

TY - JOUR

T1 - Naturally occurring parvoviral infection in Hungarian broiler flocks

AU - Palade, Elena Alina

AU - Kisary, János

AU - Benyeda, Zsófia

AU - Mándoki, Míra

AU - Balka, Gyula

AU - Jakab, Csaba

AU - Végh, Borbála

AU - Demeter, Zoltán

AU - Rusvai, M.

PY - 2011/4

Y1 - 2011/4

N2 - The major enteric disease (ED) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. Viruses from numerous families have been identified in the intestinal tracts of poultry with ED, such as Astroviridae, Coronaviridae, Reoviridae, Rotaviridae, and Parvoviridae. The objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (ChPV) and turkey parvovirus (TuPV) in Hungarian flocks experiencing clinical signs of ED. ChPV and TuPV infection were demonstrated in 15 chicken flocks and two turkey flocks, in intestinal samples collected between 2008 and 2010. The histopathological investigation revealed enteritis in the duodenum and jejunum, and atrophy of the lymphoid organs. Indirect immunohistochemistry (IHC) suggested the intestinal epithelium of chickens and turkeys as a potential replication site of the virus, similarly to other parvoviruses, while in case of the turkey samples IHC positivity was also observed in the bursa of Fabricius, liver and pancreas. However, no direct connection could be established between the presence of the pathogen in the above-mentioned tissues and the histopathological changes observed in the investigated flocks. The phylogenetic analysis performed on the partial nucleic acid sequence of the NS1 gene revealed an evident clustering tendency of the ChPV and TuPV strains, but also highlighted the potential reciprocal role of these two species in the epidemiology of these viruses. The role of the ChPV and TuPV in the ED is far from understood, but the results of the present study emphasize the fact that in certain, still not fully elucidated conditions, ChPV and TuPV may participate in the emergence of ED in chicken flocks, as suggested by previous experimental infections.

AB - The major enteric disease (ED) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. Viruses from numerous families have been identified in the intestinal tracts of poultry with ED, such as Astroviridae, Coronaviridae, Reoviridae, Rotaviridae, and Parvoviridae. The objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (ChPV) and turkey parvovirus (TuPV) in Hungarian flocks experiencing clinical signs of ED. ChPV and TuPV infection were demonstrated in 15 chicken flocks and two turkey flocks, in intestinal samples collected between 2008 and 2010. The histopathological investigation revealed enteritis in the duodenum and jejunum, and atrophy of the lymphoid organs. Indirect immunohistochemistry (IHC) suggested the intestinal epithelium of chickens and turkeys as a potential replication site of the virus, similarly to other parvoviruses, while in case of the turkey samples IHC positivity was also observed in the bursa of Fabricius, liver and pancreas. However, no direct connection could be established between the presence of the pathogen in the above-mentioned tissues and the histopathological changes observed in the investigated flocks. The phylogenetic analysis performed on the partial nucleic acid sequence of the NS1 gene revealed an evident clustering tendency of the ChPV and TuPV strains, but also highlighted the potential reciprocal role of these two species in the epidemiology of these viruses. The role of the ChPV and TuPV in the ED is far from understood, but the results of the present study emphasize the fact that in certain, still not fully elucidated conditions, ChPV and TuPV may participate in the emergence of ED in chicken flocks, as suggested by previous experimental infections.

UR - http://www.scopus.com/inward/record.url?scp=79956159462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956159462&partnerID=8YFLogxK

U2 - 10.1080/03079457.2011.553213

DO - 10.1080/03079457.2011.553213

M3 - Article

C2 - 21500039

AN - SCOPUS:79956159462

VL - 40

SP - 191

EP - 197

JO - Avian Pathology

JF - Avian Pathology

SN - 0307-9457

IS - 2

ER -