Naturally occurring and disease-associated auto-antibodies against topoisomerase I: A fine epitope mapping study in systemic sclerosis and systemic lupus erythematosus

Diána Simon, T. Czömpöly, T. Berki, Tünde Minier, Attila Peti, Eszter Tóth, L. Czirják, P. Németh

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Abstract

Auto-antibodies against topoisomerase I (topo I) are frequently detected in sera of systemic sclerosis (SSc) patients. Anti-topo I auto-antibodies are considered to be associated with the diffuse cutaneous form of systemic sclerosis (dcSSc). However, anti-topo I auto-antibodies are also detected in limited cutaneous systemic sclerosis (lcSSc) and systemic lupus erythematosus (SLE). In this study, we compared the epitope specificity of anti-topo I auto-antibodies present in sera of dcSSc, lcSSc and SLE patients. We have constructed an antigen fragment library displayed on bacteriophage lambda and screened this library with IgG purified from patients' sera. Regions of topo I selected from the library were expressed as recombinant fusion proteins and were tested with ELISA and western blot. We unexpectedly found that antibodies against a fragment of topo I {fragment F4 [amino acid (AA)] 451- 593} could be detected in sera of healthy individuals and patients with inflammatory rheumatic diseases other than SSc and SLE. Using sera of dcSSc, lcSSc and SLE patients, we showed that the pattern of recognized epitopes is different between these patient groups. Fragment F4 was recognized by all patients. Fragment F1 (AA 5-30) was recognized by 9 of 34 dcSSc patients. Fragment F8 (AA 350-400) was recognized by four of eight SLE patients. Analysis of clinical data revealed a significant difference between the F1-negative and F1-positive groups of SSc patients in age and in the duration of the disease. According to our results, the newly identified fragments F1 and F8 could represent characteristic epitopes for dcSSc and SLE, respectively.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalInternational Immunology
Volume21
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Epitope Mapping
Type I DNA Topoisomerase
Systemic Scleroderma
Systemic Lupus Erythematosus
Diffuse Scleroderma
Antibodies
Epitopes
Serum
Amino Acids
Skin
Libraries
Recombinant Fusion Proteins
Bacteriophage lambda
Rheumatic Diseases
Immunoglobulin G
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • Naturally occurring auto-antibodies
  • Phage display
  • Systemic lupus erythematosus
  • Systemic sclerosis
  • Topoisomerase I

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Naturally occurring and disease-associated auto-antibodies against topoisomerase I: A fine epitope mapping study in systemic sclerosis and systemic lupus erythematosus",
abstract = "Auto-antibodies against topoisomerase I (topo I) are frequently detected in sera of systemic sclerosis (SSc) patients. Anti-topo I auto-antibodies are considered to be associated with the diffuse cutaneous form of systemic sclerosis (dcSSc). However, anti-topo I auto-antibodies are also detected in limited cutaneous systemic sclerosis (lcSSc) and systemic lupus erythematosus (SLE). In this study, we compared the epitope specificity of anti-topo I auto-antibodies present in sera of dcSSc, lcSSc and SLE patients. We have constructed an antigen fragment library displayed on bacteriophage lambda and screened this library with IgG purified from patients' sera. Regions of topo I selected from the library were expressed as recombinant fusion proteins and were tested with ELISA and western blot. We unexpectedly found that antibodies against a fragment of topo I {fragment F4 [amino acid (AA)] 451- 593} could be detected in sera of healthy individuals and patients with inflammatory rheumatic diseases other than SSc and SLE. Using sera of dcSSc, lcSSc and SLE patients, we showed that the pattern of recognized epitopes is different between these patient groups. Fragment F4 was recognized by all patients. Fragment F1 (AA 5-30) was recognized by 9 of 34 dcSSc patients. Fragment F8 (AA 350-400) was recognized by four of eight SLE patients. Analysis of clinical data revealed a significant difference between the F1-negative and F1-positive groups of SSc patients in age and in the duration of the disease. According to our results, the newly identified fragments F1 and F8 could represent characteristic epitopes for dcSSc and SLE, respectively.",
keywords = "Naturally occurring auto-antibodies, Phage display, Systemic lupus erythematosus, Systemic sclerosis, Topoisomerase I",
author = "Di{\'a}na Simon and T. Cz{\"o}mp{\"o}ly and T. Berki and T{\"u}nde Minier and Attila Peti and Eszter T{\'o}th and L. Czirj{\'a}k and P. N{\'e}meth",
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TY - JOUR

T1 - Naturally occurring and disease-associated auto-antibodies against topoisomerase I

T2 - A fine epitope mapping study in systemic sclerosis and systemic lupus erythematosus

AU - Simon, Diána

AU - Czömpöly, T.

AU - Berki, T.

AU - Minier, Tünde

AU - Peti, Attila

AU - Tóth, Eszter

AU - Czirják, L.

AU - Németh, P.

PY - 2009

Y1 - 2009

N2 - Auto-antibodies against topoisomerase I (topo I) are frequently detected in sera of systemic sclerosis (SSc) patients. Anti-topo I auto-antibodies are considered to be associated with the diffuse cutaneous form of systemic sclerosis (dcSSc). However, anti-topo I auto-antibodies are also detected in limited cutaneous systemic sclerosis (lcSSc) and systemic lupus erythematosus (SLE). In this study, we compared the epitope specificity of anti-topo I auto-antibodies present in sera of dcSSc, lcSSc and SLE patients. We have constructed an antigen fragment library displayed on bacteriophage lambda and screened this library with IgG purified from patients' sera. Regions of topo I selected from the library were expressed as recombinant fusion proteins and were tested with ELISA and western blot. We unexpectedly found that antibodies against a fragment of topo I {fragment F4 [amino acid (AA)] 451- 593} could be detected in sera of healthy individuals and patients with inflammatory rheumatic diseases other than SSc and SLE. Using sera of dcSSc, lcSSc and SLE patients, we showed that the pattern of recognized epitopes is different between these patient groups. Fragment F4 was recognized by all patients. Fragment F1 (AA 5-30) was recognized by 9 of 34 dcSSc patients. Fragment F8 (AA 350-400) was recognized by four of eight SLE patients. Analysis of clinical data revealed a significant difference between the F1-negative and F1-positive groups of SSc patients in age and in the duration of the disease. According to our results, the newly identified fragments F1 and F8 could represent characteristic epitopes for dcSSc and SLE, respectively.

AB - Auto-antibodies against topoisomerase I (topo I) are frequently detected in sera of systemic sclerosis (SSc) patients. Anti-topo I auto-antibodies are considered to be associated with the diffuse cutaneous form of systemic sclerosis (dcSSc). However, anti-topo I auto-antibodies are also detected in limited cutaneous systemic sclerosis (lcSSc) and systemic lupus erythematosus (SLE). In this study, we compared the epitope specificity of anti-topo I auto-antibodies present in sera of dcSSc, lcSSc and SLE patients. We have constructed an antigen fragment library displayed on bacteriophage lambda and screened this library with IgG purified from patients' sera. Regions of topo I selected from the library were expressed as recombinant fusion proteins and were tested with ELISA and western blot. We unexpectedly found that antibodies against a fragment of topo I {fragment F4 [amino acid (AA)] 451- 593} could be detected in sera of healthy individuals and patients with inflammatory rheumatic diseases other than SSc and SLE. Using sera of dcSSc, lcSSc and SLE patients, we showed that the pattern of recognized epitopes is different between these patient groups. Fragment F4 was recognized by all patients. Fragment F1 (AA 5-30) was recognized by 9 of 34 dcSSc patients. Fragment F8 (AA 350-400) was recognized by four of eight SLE patients. Analysis of clinical data revealed a significant difference between the F1-negative and F1-positive groups of SSc patients in age and in the duration of the disease. According to our results, the newly identified fragments F1 and F8 could represent characteristic epitopes for dcSSc and SLE, respectively.

KW - Naturally occurring auto-antibodies

KW - Phage display

KW - Systemic lupus erythematosus

KW - Systemic sclerosis

KW - Topoisomerase I

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U2 - 10.1093/intimm/dxp008

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