Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase

Fanni S. Kálmán, Beáta Lizák, Szilvia K. Nagy, Tamás Mészáros, Veronika Zámbó, József Mandl, Miklós Csala, Éva Kereszturi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the effect of natural missense mutations on the expression of human NCB5OR. Protein and mRNA levels of five non-synonymous coding variants were analyzed in transfected HEK293 and HepG2 cells. Although the mRNA levels were only slightly affected by the mutations, the amount of Ncb5or protein was largely reduced upon two Glu to Gly replacements in the third exon (p.E87G, p.E93G). These two mutations remarkably and synergistically shortened the half-life of Ncb5or and their effect could be attenuated by proteasome inhibitors. Our results strongly indicate that p.E87G, p.E93G mutations lead to enhanced proteasomal degradation due to manifest conformational alterations in the b5 domain. These data provide first evidence for natural mutations in NCB5OR gene resulting in decreased protein levels and hence having potential implications in human pathology.

Original languageEnglish
Pages (from-to)1403-1410
Number of pages8
JournalBiochimie
Volume95
Issue number7
DOIs
Publication statusPublished - Jul 1 2013

Keywords

  • Desaturase
  • Diabetes
  • Endoplasmic reticulum
  • Missense mutation
  • Proteasome

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase'. Together they form a unique fingerprint.

  • Cite this