Natural insertions within the N-terminal region of the coat protein of Maize dwarf mosaic potyvirus (MDMV) have an effect on the RNA stability

Kathrin Petrik, Endre Sebestyén, Gyöngyvér Gell, Ervin Balázs

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A 13 amino acid residue insertion was found in the N-terminal region of the coat protein of several Maize dwarf mosaic virus isolates (MDMV). These insertions seem to be the result of a direct duplication event, but differ in some positions. In order to evaluate the influence of the insertion on the RNA secondary structure and stability, the RNA secondary structures and minimum free energies (MFE) of all existing MDMV coat protein sequences were estimated using three different softwares, the Vienna RNA Package, NUPACK, and UNAFold, and compared to the secondary structure and MFE of various random sequence collections preserving the nucleotide distribution of MDMV. The bioinformatic analysis showed that the insertion stabilizes the RNA structure of the coat protein gene.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalVirus Genes
Volume40
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

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Keywords

  • Coat protein
  • Insertion
  • Potyvirus
  • RNA secondary structure
  • RNA stability

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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