Na+/Ca2+ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa

Kogiku Shiba, T. Márián, Z. Krasznai, Shoji A. Baba, Masaaki Morisawa, Manabu Yoshida

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Ion channels and ion exchangers are known to be important participants in various aspects of sperm physiology, e.g. motility activation, chemotaxis, the maintenance of motility and the acrosome reaction in the sperm. We report here on a role of the K+-independent Na+/Ca2+ exchanger (NCX) on ascidian sperm. Reverse-transcriptase PCR reveals that the NCX is expressed in the testis while immunoblotting and immunolocalization demonstrate that the NCX exists on the sperm in the ascidian Ciona savignyi and C. intestinalis. A potent blocker of the NCX, KB-R7943 was found to block sperm-activating and -attracting factor (SAAF)-induced motility activation, sperm motility and sperm chemotaxis. We further analyzed the effects of this blocker on motility parameters such as the flagellar waveform, curvature, beat frequency, amplitude and wavelength of the sperm flagella. Inhibition of the NCX caused two distinct effects: a low concentration of KB-R7943 induced symmetric bending, whereas a high concentration of KB-R7943 resulted in asymmetric flagellar bending. These findings suggest that the NCX plays important roles in the regulation of SAAF-induced sperm chemotaxis, motility activation and motility maintenance in the ascidian. This study provides new information toward an understanding of Ca2+ transport systems in sperm motility and chemotaxis.

Original languageEnglish
Pages (from-to)623-632
Number of pages10
JournalCell Motility and the Cytoskeleton
Volume63
Issue number10
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Urochordata
Chemotaxis
Spermatozoa
Sperm Motility
Maintenance
Sperm Tail
Acrosome Reaction
Reverse Transcriptase Polymerase Chain Reaction
Ion Channels
Immunoblotting
Testis
Ions

Keywords

  • Ascidian
  • Chemotaxis
  • Flagella
  • Na/Ca exchanger
  • SAAF
  • Sperm motility

ASJC Scopus subject areas

  • Cell Biology

Cite this

Na+/Ca2+ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa. / Shiba, Kogiku; Márián, T.; Krasznai, Z.; Baba, Shoji A.; Morisawa, Masaaki; Yoshida, Manabu.

In: Cell Motility and the Cytoskeleton, Vol. 63, No. 10, 10.2006, p. 623-632.

Research output: Contribution to journalArticle

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