Nanosphere lithography as a versatile method to generate surface-imprinted polymer films for selective protein recognition

Júlia Bognár, Júlia Szucs, Zsanett Dorkõ, V. Horváth, R. Gyurcsányi

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A versatile approach based on nanosphere lithography is proposed to generate surface-imprinted polymers for selective protein recognition. A layer of 750 nm diameter latex bead-protein conjugate is deposited onto the surface of gold-coated quartz crystals followed by the electrosynthesis of a poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT/PSS) film with thicknesses on the order of the bead radius. The removal of the polymer bead-protein conjugates, facilitated by using a cleavable protein-nanosphere linkage is shown to result in 2D arrays of periodic complementary size cavities. Here it is demonstrated by nanogravimetric measurements that the imprinting proceeds further at molecular level and the protein (avidin) coating of the beads generates selective recognition sites for avidin on the surface of the PEDOT/PSS film. The binding capacity of such surface-imprinted polymer films is ca. 6.5 times higher than that of films imprinted with unmodified beads. They also exhibit excellent selectivity against analogues of avidin, i.e., extravidin, streptavidin, and neutravidin, the latter being in fact undetectable. This methodology, if coupled with properly oriented conjugation of the macromolecular template to the nanoparticles, offers the possibility of site-directed imprinting. A versatile approach based on nanosphere lithography is proposed to generate surface-imprinted polymers for selective protein recognition. Nanogravimetric measurements demonstrate that the protein (avidin) coating of the nanospheres generates selective recognition sites for avidin on the surface of the PEDOT/PSS film. This methodology coupled with oriented conjugation of the macromolecular template to the nanospheres offers the possibility of site-directed imprinting.

Original languageEnglish
Pages (from-to)4703-4709
Number of pages7
JournalAdvanced Functional Materials
Volume23
Issue number37
DOIs
Publication statusPublished - Oct 4 2013

Fingerprint

Nanospheres
Polymer films
Lithography
Avidin
lithography
beads
proteins
Proteins
polymers
Polymers
conjugation
templates
methodology
coatings
Coatings
electrochemical synthesis
Quartz
Streptavidin
Latex
latex

Keywords

  • molecularly imprinted polymers
  • nanosphere lithography
  • PEDOT/PSS
  • protein recognition
  • surface imprinting

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Nanosphere lithography as a versatile method to generate surface-imprinted polymer films for selective protein recognition. / Bognár, Júlia; Szucs, Júlia; Dorkõ, Zsanett; Horváth, V.; Gyurcsányi, R.

In: Advanced Functional Materials, Vol. 23, No. 37, 04.10.2013, p. 4703-4709.

Research output: Contribution to journalArticle

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