Nanosilver pathophysiology in earthworms: Transcriptional profiling of secretory proteins and the implication for the protein corona

Yuya Hayashi, Teodora Miclaus, P. Engelmann, Herman Autrup, Duncan S. Sutherland, Janeck J. Scott-Fordsmand

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previously we have identified lysenin as a key protein constituent of the secretome from Eisenia fetida coelomocytes and revealed its critical importance in priming interactions between the cells and the protein corona around nanosilver. As alterations of the protein environment can directly affect the corona composition, the extent to which nanoparticles influence the cells protein secretion profile is of remarkable interest that has rarely acquired attention. Here, we have probed transcriptional responses of E. fetida coelomocytes to the representative nanosilver NM-300K (15 nm) in a time-dependent manner (2, 4, 8 and 24 h at a low-cytotoxic concentration), and examined the implication of the temporal changes in transcriptional profiles of secretory proteins with a particular reference to that of lysenin. NM-300K was accumulated in/at the cells and lysenin was, after transient induction, gradually suppressed over time indicating a negative feedback cycle. This may limit further enrichment of lysenin in the corona and thereby decrease the lysenin-assisted uptake of the nanoparticles. Other differentially expressed genes were those involved in metal stress (likewise in AgNO3-stressed cells) and in Toll-like receptor (TLR) signaling. This offers an intriguing perspective of the nanosilver pathophysiology in earthworms, in which the conserved pattern recognition receptor TLRs may play an effector role.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalNanotoxicology
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 15 2016

Fingerprint

Oligochaeta
Proteins
Nanoparticles
Pattern Recognition Receptors
Toll-Like Receptors
Cell Communication
Pattern recognition
Genes
Metals
lysenin
Protein Corona
Feedback
Chemical analysis

Keywords

  • Biomolecular corona
  • coelomocytes
  • gene expression
  • NM-300K
  • toll-like receptors

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

Nanosilver pathophysiology in earthworms : Transcriptional profiling of secretory proteins and the implication for the protein corona. / Hayashi, Yuya; Miclaus, Teodora; Engelmann, P.; Autrup, Herman; Sutherland, Duncan S.; Scott-Fordsmand, Janeck J.

In: Nanotoxicology, Vol. 10, No. 3, 15.03.2016, p. 303-311.

Research output: Contribution to journalArticle

Hayashi, Yuya ; Miclaus, Teodora ; Engelmann, P. ; Autrup, Herman ; Sutherland, Duncan S. ; Scott-Fordsmand, Janeck J. / Nanosilver pathophysiology in earthworms : Transcriptional profiling of secretory proteins and the implication for the protein corona. In: Nanotoxicology. 2016 ; Vol. 10, No. 3. pp. 303-311.
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