Nanoparticles for intravascular applications: Pcharacterization and cytotoxicity testing

Jasmin Matuszak, Jens Baumgartner, Jan Zaloga, Maya Juenet, Acarília Eduardo Da Silva, Danielle Franke, Gunter Almer, Isabelle Texier, Damien Faivre, Josbert M. Metselaar, Fabrice P. Navarro, Cédric Chauvierre, Ruth Prassl, László Dézsi, Rudolf Urbanics, Christoph Alexiou, Harald Mangge, János Szebeni, Didier Letourneur, Iwona Cicha

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

Original languageEnglish
Pages (from-to)597-616
Number of pages20
JournalNanomedicine
Volume11
Issue number6
DOIs
Publication statusPublished - Mar 2016

    Fingerprint

Keywords

  • atherosclerosis
  • endothelial cells
  • hypersensitivity reaction
  • live-cell analysis
  • nanoparticle biocompatibility
  • nanoparticle stability

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Matuszak, J., Baumgartner, J., Zaloga, J., Juenet, M., Da Silva, A. E., Franke, D., Almer, G., Texier, I., Faivre, D., Metselaar, J. M., Navarro, F. P., Chauvierre, C., Prassl, R., Dézsi, L., Urbanics, R., Alexiou, C., Mangge, H., Szebeni, J., Letourneur, D., & Cicha, I. (2016). Nanoparticles for intravascular applications: Pcharacterization and cytotoxicity testing. Nanomedicine, 11(6), 597-616. https://doi.org/10.2217/nnm.15.216